Sp1 and VEGF mRNA expression in the retina micrangium endothelial cells of streptozotocin-induced diabetic rats

被引：0

作者：

Li, En-Hui ^{[2
]}

Lai, Xiao-Li ^{[1
]}

Xiang, Zhen-Yang ^{[2
]}

Lin, Xian-Ping ^{[2
]}

Cui, Gang-Feng ^{[2
]}

Li, Gao-Chun ^{[2
]}

机构：

[1] Taizhou Hosp Zhejiang Prov, Wenzhou Med Coll, Dept Obstet & Gynaecol, Linhai, Zhejiang, Peoples R China

[2] Taizhou Hosp Zhejiang Prov, Wenzhou Med Coll, Dept Ophthalmol, Linhai, Zhejiang, Peoples R China

来源：

AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH | 2012年 / 6卷 / 03期

关键词：

Sp1; VEGF; PCR; Retina; Immunohistochemistry; TRANSCRIPTION FACTOR SP1; PROMOTER IN-VIVO; GENE-EXPRESSION; MYELOID CELLS; GROWTH-FACTOR; MUSCLE-CELLS; SITES; RETINOPATHY; GLUCOSE; ACTIVATION;

D O I：

10.5897/AJMR11.1271

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The aim of this study is to evaluate the Sp1 and VEGF mRNA expression in the retina micrangium endothelial cells of streptozotocin-induced diabetic rats. At the end of our study, we determined that SP1 positive expression rate and the Sp1, VEGF mRNA and protein expression in the retina micrangium endothelial cells in isolated rat eyes of diabetic rats were significantly increased compared to normal animals. The ERK, Sp1 and VEGF protein expression in the retina micrangium endothelial cells of diabetic rats was significantly decreased in high-surcose (Sp1 specific inhibitor Mithramycin A or ERK specific inhibitor PD98059) culture group in comparison to normal (vechile PBS) culture group. These findings suggest that ERK, Sp1 and VEGF protein expression is an important factor to affect the occurrence and development of vision diseases in diabetic patients.

引用

页码：624 / 628

页数：5

共 23 条

[1]

Akiyama H, 2002, INVEST OPHTH VIS SCI, V43, P1367

[2] Diabetic retinopathy - Seeing beyond glucose-induced microvascular disease [J].