Uric acid metabolism of kidney and intestine in a rat model of chronic kidney disease

被引：28
作者：
Nagura, Michito [1 ]
Tamura, Yoshifuru [1 ]
Kumagai, Takanori [1 ,2 ]
Hosoyamada, Makoto [3 ]
Uchida, Shunya [1 ]
机构：
[1] Teikyo Univ, Sch Med, Dept Internal Med, Tokyo, Japan
[2] Teikyo Univ, Sch Med, Support Community Med Endowed Chair, Tokyo, Japan
[3] Teikyo Univ, Fac Pharma Sci, Human Physiol & Pathol, Tokyo, Japan
来源：
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2016年 / 35卷 / 10-12期
关键词：
Chronic kidney disease; remnant kidney model; creatinine clearance; urate transporter; ABCG; intestinal uric acid excretion; oxonic acid; RESISTANCE PROTEIN TRANSPORTER; RENAL-INSUFFICIENCY; RISK; ABCG2;
D O I：
10.1080/15257770.2016.1163379
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Uric acid (UA) is a potential risk factor of the progression of chronic kidney disease (CKD). Recently, we reported that intestinal UA excretion might be enhanced via upregulation of the ATP-binding cassette transporter G2 (Abcg2) in a 5/6 nephrectomy (Nx) rat model. In the present study, we examined the mRNA and protein expressions of UA transporters, URAT1, GLUT9/URATv1, ABCG2 and NPT4 in the kidney and ileum in the same rat model. Additionally, we investigated the Abcg2 mRNA expression of ileum in hyperuricemic rat model by orally administering oxonic acid. Male Wistar rats were randomly assigned to three groups consisting of Nx group, oxonic acid-treated (Ox) group and sham-operated control group, and sacrificed at 8 weeks. Creatinine and UA were measured and them RNA expressions of UA transporters in the kidney and intestine were evaluated by a real time PCR. UA transporters in the kidney sections were also examined by immunohistochemistry. Serum creatinine elevated in the Nx group whereas serum UA increased in the Ox group. Both the mRNA expression and the immunohistochemistry of the UA transporters were decreased in the Nx group, suggesting a marginal role in UA elevation in decreased kidney function. In contrast, the mRNA expression of Abcg2 in the ileum significantly increased in the Ox group. These results suggest that the upregulation of Abcg2 mRNA in the ileum triggered by an elevation of serum UA may play a compensatory role in increasing intestinal UA excretion.
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收藏
页码：550 / 558
页数：9
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