Inhibitory Mechanism of Taxifolin against α-Glucosidase Based on Spectrofluorimetry and Molecular Docking

被引：0
作者：
Liu, Jiang [1 ,2 ]
Wang, Xiansheng [3 ]
Geng, Sheng [1 ]
Liu, Benguo [1 ]
Liang, Guizhao [2 ]
机构：
[1] Henan Inst Sci & Technol, Sch Food Sci, Xinxiang 453003, Peoples R China
[2] Chongqing Univ, Key Lab Biorheol Sci & Technol, Minist Educ, Bioengn Coll, Chongqing 400044, Peoples R China
[3] Jiangsu Acad Agr Sci, Inst Food Crops, Minist Agr, Nanjing Stn,DUS Testing Ctr New Plant Varieties, Nanjing 210014, Jiangsu, Peoples R China
来源：
NATURAL PRODUCT COMMUNICATIONS | 2017年 / 12卷 / 11期
基金：
中国国家自然科学基金;
关键词：
Taxifolin; alpha-Glucosidase; Inhibition mechanism; Spectrofluorimetry; Molecular docking; IN-VITRO; DIHYDROQUERCETIN; PREVENTION;
D O I：
暂无
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
The alpha-glucosidase inhibitory activity and behavior of taxifolin was first investigated by spectrofluorimetry and molecular docking. It was found that taxifolin inhibits alpha-glucosidase in a competitive manner with the IC50 value of 0.16 mg/mL. The intrinsic fluorescence quenching of alpha-glucosidase in the presence of taxifolin was observed by the static quenching mechanism. According to the thermodynamic study, the complex of taxifolin and alpha-glucosidase was maintained by van der Waals and hydrogen bonding. The binding mode provided by molecular docking simulation indicated the existence of hydrogen bonding between taxifolin and the amino acid residues of a-glucosidase (Glu429, Asp 568 and Glu771), which coincided with the result of fluorescence analysis.
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页码：1725 / 1728
页数：4
相关论文
共 18 条
[1]  
Aminudin NI, 2015, NAT PROD COMMUN, V10, P1585
[2]   Constituent analysis of the ethanol extracts of Chimonanthus nitens Oliv. leaves and their inhibitory effect on α-glucosidase activity [J].
Hui, Chen ;
Ouyang, Kehui ;
Yan, Jiang ;
Yang, Zhanwei ;
Hu, Wenbing ;
Lei, Xiong ;
Ning, Wang ;
Xin, Liu ;
Wang, Wenjun .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2017, 98 :829-836
[3]   Spectroscopic and molecular modeling studies of human serum albumin interaction with propyl gallate [J].
Dolatabadi, Jafar Ezzati Nazhad ;
Panahi-Azar, Vahid ;
Barzegar, Abolfazl ;
Jamali, Ali Akbar ;
Kheirdoosh, Fahimeh ;
Kashanian, Soheila ;
Omidi, Yadollah .
RSC ADVANCES, 2014, 4 (110) :64559-64564
[4]   α-Glucosidase and α-amylase inhibitors from Myrcia spp.: a stronger alternative to acarbose? [J].
Figueiredo-Gonzalez, Maria ;
Grosso, Clara ;
Valentao, Patricia ;
Andrade, Paula B. .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2016, 118 :322-327
[5]   Molecular Docking and Inhibition Kinetics of α-glucosidase Activity by Labdane Diterpenes Isolated from Tora Seeds (Alpinia nigra BL Burtt.) [J].
Ghosh, Sudipta ;
Rangan, Latha .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2015, 175 (03) :1477-1489
[6]   Acarbose Monotherapy and Type 2 Diabetes Prevention in Eastern and Western Prediabetes: An Ethnicity-specific Meta-analysis [J].
Hu, Ruijie ;
Li, Yi ;
Lv, Qingguo ;
Wu, Taixiang ;
Tong, Nanwei .
CLINICAL THERAPEUTICS, 2015, 37 (08) :1798-1812
[7]   Adverse childhood experiences and risk of type 2 diabetes: A systematic review and meta-analysis [J].
Huang, Hao ;
Yan, Peipei ;
Shan, Zhilei ;
Chen, Sijing ;
Li, Moying ;
Luo, Cheng ;
Gao, Hui ;
Hao, Liping ;
Liu, Liegang .
METABOLISM-CLINICAL AND EXPERIMENTAL, 2015, 64 (11) :1408-1418
[8]   Spectroscopic investigations of the interaction of the anti-hypertension drug valsartan with human serum albumin [J].
Jing, Jian ;
Qu, Xin ;
Tu, Zhi ;
Zheng, Chenhoo ;
Zheng, Zhicheng .
MOLECULAR MEDICINE REPORTS, 2014, 9 (06) :2191-2196
[9]   In vitro inhibition of α-glucosidases and glycogen phosphorylase by catechin gallates in green tea [J].
Kamiyama, Ogusa ;
Sanae, Fujiko ;
Ikeda, Kyoko ;
Higashi, Yasuhiko ;
Minami, Yasuhiro ;
Asano, Naoki ;
Adachi, Isao ;
Kato, Atsushi .
FOOD CHEMISTRY, 2010, 122 (04) :1061-1066
[10]   Laccase-mediated biotransformation of dihydroquercetin (taxifolin) [J].
Khlupova, Maria ;
Vasil'eva, Irina ;
Shumakovich, Galina ;
Morozova, Olga ;
Chertkov, Vyacheslav ;
Shestakova, Alla ;
Kisinc, Alexander ;
Yaropolov, Alexander .
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2016, 123 :62-66
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