Evidence for the involvement of cofilin in Aspergillus fumigatus internalization into type II alveolar epithelial cells

被引：22

作者：

Bao, Zhiyao ^{[1
,3
]}

Han, Xuelin ^{[2
]}

Chen, Fangyan ^{[2
]}

Jia, Xiaodong ^{[2
]}

Zhao, Jingya ^{[2
]}

Zhang, Changjian ^{[2
]}

Yong, Chen ^{[2
]}

Tian, Shuguang ^{[2
]}

Zhou, Xin ^{[1
]}

Han, Li ^{[2
]}

机构：

[1] Shanghai Jiao Tong Univ, Dept Resp Med, Shanghai Peoples Hosp 1, Shanghai 200080, Peoples R China

[2] Acad Mil Med Sci, Dept Hosp Infect Control & Res, Inst Dis & Prevent PLA, Beijing 100071, Peoples R China

[3] Shanghai Jiao Tong Univ, Dept Resp Med, Ruijin Hosp, Sch Med, Shanghai 200025, Peoples R China

来源：

BMC MICROBIOLOGY | 2015年 / 15卷

关键词：

Aspergillus fumigatus; Internalization; Cofilin; Lung epithelial cell;

D O I：

10.1186/s12866-015-0500-y

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Background: The internalization of Aspergillus fumigatus into alveolar epithelial cells (AECs) is tightly controlled by host cellular actin dynamics, which require close modulation of the ADF (actin depolymerizing factor)/cofilin family. However, the role of cofilin in A. fumigatus internalization into AECs remains unclear. Results: Here, we demonstrated that germinated A. fumigatus conidia were able to induce phosphorylation of cofilin in A549 cells during the early stage of internalization. The modulation of cofilin activity by overexpression, knockdown, or mutation of the cofilin gene in A549 cells decreased the efficacy of A. fumigatus internalization. Reducing the phosphorylation status of cofilin with BMS-5 (LIM kinase inhibitor) or overexpression of the slingshot phosphatases also impeded A. fumigatus internalization. Both the C. botulimun C3 transferase (a specific RhoA inhibitor) and Y27632 (a specific ROCK inhibitor) reduced the internalization of A. fumigatus and the level of phosphorylated cofilin. beta-1,3-glucan (the major component of the conidial cell wall) and its host cell receptor dectin-1 did not seem to be associated with cofilin phosphorylation during A. fumigatus infection. Conclusion: These results indicated that cofilin might be involved in the modulation of A. fumigatus internalization into type II alveolar epithelial cells through the RhoA-ROCK-LIM kinase pathway.

引用

页数：11

共 35 条

[1]

Beisswenger C., Hess C., Bals R., Aspergillus fumigatus conidia induce interferon-β signalling in respiratory epithelial cells, Eur Respir J, 39, pp. 411-418, (2012)

[2]

Paris S., Boisvieux-Ulrich E., Crestani B., Houcine O., Taramelli D., Lombardi L., Et al., Internalization of Aspergillus fumigatus conidia by epithelial and endothelial cells, Infect Immun, 65, pp. 1510-1514, (1997)

[3]

Wasylnka J.A., Moore M.M., Aspergillus fumigatus conidia survive and germinate in acidic organelles of A549 epithelial cells, J Cell Sci, 116, pp. 1579-1587, (2003)

[4]

Ghosh M., Song X., Mouneimne G., Sidani M., Lawrence D.S., Condeelis J.S., Cofilin promotes actin polymerization and defines the direction of cell motility, Science, 304, pp. 743-746, (2004)

[5]

Bernstein B.W., Bamburg J.R., ADF/cofilin: A functional node in cell biology, Trends Cell Biol, 20, pp. 187-195, (2010)

[6]

Van Troys M., Huyck L., Leyman S., Dhaese S., Vandekerkhove J., Ampe C., Ins and outs of ADF/cofilin activity and regulation, Eur J Cell Biol, 87, pp. 649-667, (2008)

[7]

Bravo-Cordero J.J., Magalhaes M.A., Eddy R.J., Hodgson L., Condeelis J., Functions of cofilin in cell locomotion and invasion, Nat Rev Mol Cell Biol, 14, 7, pp. 405-415, (2013)

[8]

Welch M.D., Way M., Arp2/3-mediated actin-based motility: A tail of pathogen abuse, Cell Host Microbe, 14, 3, pp. 242-255, (2013)

[9]

Mizuno K., Signalling mechanisms and functional roles of cofilin phosphorylation and dephosphorylation, Cell Signalling, 25, pp. 457-469, (2013)

[10]

Scott R.W., Olson M.F., LIM kinases: Function, regulation and association with human disease, J Mol Med (Berl), 85, pp. 555-568, (2007)

← 1 2 3 4 →