Development of Thiabendazole-Loaded Mesoporous Silica Nanoparticles for Cancer Therapy

被引:23
作者
Esfahani, Maedeh Koohi Moftakhari [1 ,2 ]
Islam, Nazrul [3 ]
Cabot, Peter J. [4 ]
Izake, Emad L. [1 ,2 ,4 ]
机构
[1] Queensland Univ Technol QUT, Sci & Engn Fac, Sch Chem & Phys, Brisbane, Qld 4000, Australia
[2] Queensland Univ Technol QUT, Ctr Mat Sci, Brisbane, Qld 4000, Australia
[3] Queensland Univ Technol, Fac Hlth, Sch Clin Sci, Brisbane, Qld 4000, Australia
[4] Univ Queensland, Sch Pharm, Woolloongabba, Qld 4102, Australia
关键词
cancer; drug delivery; MCM-41; mesoporous silica nanoparticles; thiabendazole; ANTHELMINTIC DRUG MEBENDAZOLE; IN-VITRO; CONTROLLED-RELEASE; DELIVERY-SYSTEMS;
D O I
10.1021/acsbiomaterials.1c00066
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
Thiabendazole (TBZ) is an anthelmintic drug currently studied for anticancer purposes. However, due to its low solubility, its biomedical application has been limited. Using mesoporous silica nanoparticles (MSNPs), such as Mobil Composition of Matter Number 41 (MCM-41), as a drug carrier, is a promising approach to improve the solubility of low water-soluble drugs. In the present work, we aim to develop TBZ-loaded MCM-41 (TBZ MCM-41) nanoparticles to improve the solubility and the therapeutic efficacy of TBZ against prostate cancer PC-3 cells. TBZ MCM-41 nanoparticles were synthesized with a size of 215.9 +/- 0.07 nm, a spherical shape, a hexagonal array of channels, and a drug loading capacity of 19.1%. The biological effects of the nanoformulation on PC-3 cells were then evaluated using a 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT), IncuCyte live-cell imaging system, cell migration, and reactive oxygen species (ROS) assays. The results demonstrated that TBZ was released from MCM-41 nanoparticles in a controlled manner at pH values of 1.2 and 6.8. The cell viability measurements revealed that the TBZ MCM-41 nanoparticles caused a considerable 2.8-fold increase in the cytotoxicity of TBZ (IC50 127.3 and 46 mu M for TBZ and TBZ MCM-41 nanoparticles, respectively). The results of the proliferation assay were in agreement with those of the cell viability measurements, where the MCM-41 increased the cytotoxicity of TBZ in a concentration-dependent manner. Also, the TBZ MCM-41 nanoparticles were found to enhance the potency of the drug and inhibit PC-3 cell migration. In addition, the ROS assay confirmed that TBZ MCM-41 nanoparticles were approximately 15% more potent than TBZ to produce ROS. Overall, the results demonstrated that MCM-41 nanoparticles are a promising carrier to improve the therapeutic efficacy of TBZ against PC-3 cells and suggest evaluating the efficacy of the formulation in vivo.
引用
收藏
页码:4153 / 4162
页数:10
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