Microplastics Reduce Lipid Digestion in Simulated Human Gastrointestinal System

被引:162
作者
Tan, Huiwen [1 ,2 ]
Yue, Tongtao [1 ]
Xu, Yan [3 ]
Zhao, Jian [1 ,2 ]
Xing, Baoshan [4 ]
机构
[1] Ocean Univ China, Inst Adv Ocean Study, Inst Coastal Environm Pollut Control, Key Lab Marine Environm & Ecol,Minist Educ, Qingdao 266100, Peoples R China
[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China
[3] Shandong Univ Sci & Technol, Coll Elect Engn & Automat, Qingdao 266590, Peoples R China
[4] Univ Massachusetts, Stockbridge Sch Agr, Amherst, MA 01003 USA
基金
中国国家自然科学基金;
关键词
NORTH-SEA; LIPASE; POLYSTYRENE; EXPOSURE;
D O I
10.1021/acs.est.0c02608
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Microplastics (MPs) are unavoidably ingested by humans, and their gastrointestinal processes and impact on lipid digestion are unknown. In the present work, all five MP types used, including polystyrene (PS), polyethylene terephthalate, polyethylene, polyvinyl chloride, and poly(lactic-co-glycolic acid) (80 mg/L in small intestine), significantly reduced lipid digestion in the in vitro gastrointestinal system. PS MPs exhibited the highest inhibition (12.7%) among the five MPs. Lipid digestion decreased with increasing PS concentration, but independent of PS size (50 nm, 1 mu m, 10 mu m). PS MPs after photoaging by simulated sunlight also significantly decreased lipid digestion. Confocal imaging shows that PS MPs could interact with both lipid droplets and lipases. Two mechanisms underlying the PS-induced digestion inhibition were revealed using both experimental and molecular dynamics simulation approaches: (1) PS MPs decreased the bioavailability of lipid droplets via forming large lipid-MPs heteroaggregates due to the high MP hydrophobicity; and (2) PS MPs adsorbed lipase, and reduced its activity by changing the secondary structure and disturbing the essential open conformation. The first mechanism (MP-lipid interaction) played a more important role in lipid digestion reduction based on interaction energy calculation. These findings reveal potential risk of MPs to human digestion health and nutrient assimilation.
引用
收藏
页码:12285 / 12294
页数:10
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