Role of Ca2+-independent phospholipase A2 in exocytosis of amylase from parotid acinar cells

We evaluated the role of cytosolic phospholipase A2 (PLA2) in the exocytosis of amylase from parotid acinar cells, The exocytosis stimulated by isoproterenol was dose-dependently inhibited by bromoenol lactone (EEL), a potent suicide inhibitor of Ca2+-independent cytosolic PLA2. The IC50 value of EEL was approximately 7 mu M. AACOCF3, a selective inhibitor of Ca2+-dependent cytosolic PLA2, did not inhibit the exocytosis at least up to 30 mu M. EEL also inhibited amylase release evoked by forskolin and membrane-permeable cAMP, but it did not inhibit cAMP-dependent protein kinase activity, PLA2 activity in parotid acinar cells was found to be predominantly Ca2+-independent, and was strongly inhibited by EEL, whose IC50 value was approximately 2 mu M when it was applied to intact acini, Although isoproterenol scarcely enhanced [H-3]arachidonic acid release from intact acinar cells, EEL dose-dependently decreased the basal arachidonic acid release to approximately one half of the control value, These results suggest that the cytosolic Ca2+-independent PLA2 activity plays a role in the membrane fusion process of exocytosis in parotid acinar cells.