The Curcumin Analogue, MS13 (1,5-Bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one), Inhibits Cell Proliferation and Induces Apoptosis in Primary and Metastatic Human Colon Cancer Cells

被引：19

作者：

Ismail, Nor Isnida ^{[1
,2
]}

Othman, Iekhsan ^{[1
]}

Abas, Faridah ^{[3
,4
]}

H. Lajis, Nordin ^{[3
]}

Naidu, Rakesh ^{[1
]}

机构：

[1] Monash Univ Malaysia, Jeffrey Cheah Sch Med & Hlth Sci, Jalan Lagoon Selatan, Bandar Sunway 47500, Selangor, Malaysia

[2] UniKL MESTECH, A1-1 Jalan TKS1, Taman Kajang Sentral 43000, Kajang, Malaysia

[3] Univ Putra Malaysia, Lab Nat Prod, Fac Sci, Serdang 43400, Malaysia

[4] Univ Putra Malaysia, Fac Food Sci & Technol, Dept Food Sci, Serdang 43400, Malaysia

来源：

MOLECULES | 2020年 / 25卷 / 17期

关键词：

curcumin analogue; diarylpentanoid (DAP); colon cancer; cell proliferation; apoptosis; 1; 5-bis(4-hydroxy-3-methoxyphenyl)-1; 4-pentadiene-3-one; Bcl-2; Caspase-3; GROWTH-SUPPRESSIVE ACTIVITY; NF-KAPPA-B; COLORECTAL-CANCER; BIOLOGICAL EVALUATION; ANTICANCER EFFICACY; IN-VITRO; MEDIATED APOPTOSIS; ANTITUMOR AGENTS; ROS PRODUCTION; CYCLE ARREST;

D O I：

10.3390/molecules25173798

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cytotoxic and apoptotic effects of turmeric (Curcuma longa) on colon cancer have been well documented but specific structural modifications of curcumin have been shown to possess greater growth-suppressive potential on colon cancer than curcumin. Therefore, the aim of this study is to identify the anti-cancer properties of curcumin analogue-MS13, a diarylpentanoid on the cytotoxicity, anti-proliferative and apoptotic activity of primary (SW480) and metastatic (SW620) human colon cancer cells. A cell viability assay showed that MS13 has greater cytotoxicity effect on SW480 (EC50: 7.5 +/- 2.8 mu M) and SW620 (EC50: 5.7 +/- 2.4 mu M) compared to curcumin (SW480, EC50: 30.6 +/- 1.4 mu M) and SW620, EC50: 26.8 +/- 2.1 mu M). Treatment with MS13 at two different doses 1X EC(50)and 2X EC(50)suppressed the colon cancer cells growth with lower cytotoxicity against normal cells. A greater anti-proliferative effect was also observed in MS13 treated colon cancer cells compared to curcumin at 48 and 72 h. Subsequent analysis on the induction of apoptosis showed that MS13 treated cells exhibited morphological features associated with apoptosis. The findings are also consistent with cellular apoptotic activities shown by increased caspase-3 activity and decreased Bcl-2 protein level in both colon cancer cell lines. In conclusion, MS13 able to suppress colon cancer cell growth by inhibiting cell proliferation and induce apoptosis in primary and metastatic human colon cancer cells.

引用

页数：23