Time-resolved spectroscopic imaging reveals the fundamentals of cellular NADH fluorescence

被引：65

作者：

Li, Dong ^{[1
]}

Zheng, Wei ^{[1
]}

Qu, Jianan Y. ^{[1
]}

机构：

[1] Hong Kong Univ Sci & Technol, Dept Elect & Comp Engn, Kowloon, Hong Kong, Peoples R China

来源：

OPTICS LETTERS | 2008年 / 33卷 / 20期

关键词：

D O I：

10.1364/OL.33.002365

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A time-resolved spectroscopic imaging system is built to study the fluorescence characteristics of nicotinamide adenine dinucleotide (NADH), an important metabolic coenzyme and endogenous fluorophore in cells. The system provides a unique approach to measure fluorescence signals in different cellular organelles and cytoplasm. The ratios of free over protein-bound NADH signals in cytosol and nucleus are slightly higher than those in mitochondria. The mitochondrial fluorescence contributes about 70% of overall cellular fluorescence and is not a completely dominant signal. Furthermore, NADH signals in mitochondria, cytosol, and the nucleus respond to the changes of cellular activity differently, suggesting that cytosolic and nuclear fluorescence may complicate the well-known relationship between mitochondrial fluorescence and cellular metabolism. (C) 2008 Optical Society of America

引用

页码：2365 / 2367

页数：3

共 13 条

[1]

BARBAN S, 1961, J BIOL CHEM, V236, P1887

[2] Optical biopsy of cytokeratin and NADH in the tumor border zone [J].

Beuthan, J ;

Minet, O ;

Müller, G .

ADVANCES IN OPTICAL BIOPSY AND OPTICAL MAMMOGRAPHY, 1998, 838 :150-170

[3] Metabolic mapping of MCF10A human breast cells via multiphoton fluorescence lifetime imaging of the coenzyme NADH [J].

Bird, DK ;

Yan, L ;

Vrotsos, KM ;

Eliceiri, KW ;

Vaughan, EM ;

Keely, PJ ;

White, JG ;

Ramanujam, N .

CANCER RESEARCH, 2005, 65 (19) :8766-8773

[4]

Campbell M K., 1995, Biochemistry, V2nd

[5] Autofluorescence properties of isolated rat hepatocytes under different metabolic conditions [J].

Croce, Anna Cleta ;

Ferrigno, Andrea ;

Vairetti, Mariapia ;

Bertone, Roberta ;

Freitas, Isabel ;

Bottiroli, Giovanni .

PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2004, 3 (10) :920-926

[6] Differential binding of NAD+ and NADH allows the transcriptional corepressor carboxyl-terminal binding protein to serve as a metabolic sensor [J].

Fjeld, CC ;

Birdsong, WT ;

Goodman, RH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (16) :9202-9207

[7] Two-photon fluorescence spectroscopy and microscopy of NAD(P)H and flavoprotein [J].

Huang, SH ;

Heikal, AA ;

Webb, WW .

BIOPHYSICAL JOURNAL, 2002, 82 (05) :2811-2825

[8] In vivo multiphoton fluorescence lifetime imaging of protein-bound and free nicotinamide adenine dinucleotide in normal and precancerous epithelia [J].

Skala, Melissa C. ;

Riching, Kristin M. ;

Bird, Damian K. ;

Gendron-Fitzpatrick, Annette ;

Eickhoff, Jens ;

Eliceiri, Kevin W. ;

Keely, Patricia J. ;

Ramanujam, Nirmala .

JOURNAL OF BIOMEDICAL OPTICS, 2007, 12 (02)

[9]

VONZGLINICKI T, 1992, J CELL SCI, V103, P1073

[10] Fluorescence proteins, live-cell imaging, and mechanobiology: Seeing is believing [J].

Wang, Yingxiao ;

Shyy, John Y. J. ;

Chien, Shu .

ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2008, 10 :1-38

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