Therapeutic Effect of the Tuber of Alisma orientale on Lipopolysaccharide-Induced Acute Lung Injury

被引：11

作者：

Kim, Kyun Ha ^{[1
]}

Kwun, Min Jung ^{[1
]}

Choi, Jun-Yong ^{[1
,2
]}

Ahn, Kyung-Seop ^{[3
]}

Oh, Sei-Ryang ^{[3
]}

Lee, Yong Gyu ^{[4
,5
]}

Christman, John W. ^{[4
,5
]}

Sadikot, Ruxana T. ^{[6
,7
]}

Han, Chang Woo ^{[1
,2
,8
]}

Joo, Myungsoo ^{[1
,9
]}

机构：

[1] Pusan Natl Univ, Sch Korean Med, Yangsan 626870, South Korea

[2] Pusan Natl Univ, Korean Med Hosp, Yangsan 626870, South Korea

[3] Korea Res Inst Biosci & Biotechnol, Immune Modulator Res Ctr, Ochang 363883, Chungbuk, South Korea

[4] Univ Illinois, Sect Pulm Crit Care & Sleep Med, Chicago, IL 60612 USA

[5] Jesse Brown Vet Affairs Med Ctr, Chicago, IL 60612 USA

[6] Univ Florida, Coll Med, Div Allergy Pulm Crit Care & Sleep Med, Gainesville, FL 32610 USA

[7] Malcom Randall VAMC, Gainesville, FL 32610 USA

[8] Pusan Natl Univ, Sch Korean Med, Dept Internal Med, Yangsan 626870, South Korea

[9] Pusan Natl Univ, Sch Korean Med, Div Appl Med, Yangsan 626870, South Korea

来源：

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2013年 / 2013卷

基金：

新加坡国家研究基金会;

关键词：

NF-KAPPA-B; RESPIRATORY-DISTRESS-SYNDROME; NRF2 ENHANCES SUSCEPTIBILITY; SMOKE-INDUCED EMPHYSEMA; RHIZOMA DRIED RHIZOME; TERPENE COMPONENTS; METHANOL EXTRACT; INFLAMMATION; ENDOTOXIN; PATHWAY;

D O I：

10.1155/2013/863892

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Although Alisma orientale, an ethnic herb, has been prescribed for treating various diseases in Asian traditional medicine, experimental evidence to support its therapeutic effects is lacking. Here, we sought to determine whether A. orientale has a therapeutic effect on acute lung injury (ALI). Ethanol extract of the tuber of A. orientale (EEAO) was prepared and fingerprinted by HPLC for its constituents. Mice received an intraperitoneal (i.p.) injection of lipopolysaccharide (LPS) for the induction of ALI. At 2 h after LPS treatment, mice received an intratracheal (i.t.) spraying of various amounts of EEAO to the lung. Bioluminescence imaging of transgenic NF-kappa B/luciferase reporter mice shows that i.t. EEAO posttreatment suppressed lung inflammation. In similar experiments with C57BL/6 mice, EEAO posttreatment significantly improved lung inflammation, as assessed by H&E staining of lung sections, counting of neutrophils in bronchoalveolar lavage fluid, and semiquantitative RT-PCR analyses of proinflammatory cytokines and Nrf2-dependent genes in the inflamed lungs. Furthermore, EEAO posttreatment enhanced the survival of mice that received a lethal dose of LPS. Together, our results provide evidence that A. orientale has a therapeutic effect on ALI induced by sepsis.

引用

页数：10

共 42 条

[31] Effects of sesquiterpenes and triterpenes from the rhizome of Alisma orientale on nitric oxide production in lipopolysaccharide-activated macrophages:: Absolute stereostructures of alismaketones-B 23-acetate and -C 23-acetate [J].