Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer
被引:3
作者:
Nakles, Rebecca E.
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机构:
Georgetown Univ, Dept Oncol, Washington, DC 20057 USAGeorgetown Univ, Dept Oncol, Washington, DC 20057 USA
Nakles, Rebecca E.
[1
]
Millman, Sarah L.
论文数: 0引用数: 0
h-index: 0
机构:
Georgetown Univ, Dept Oncol, Washington, DC 20057 USAGeorgetown Univ, Dept Oncol, Washington, DC 20057 USA
Millman, Sarah L.
[1
]
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机构:
Cabrera, M. Carla
[1
]
Johnson, Peter
论文数: 0引用数: 0
h-index: 0
机构:
Georgetown Univ, Dept Oncol, Washington, DC 20057 USA
Georgetown Univ, Lombardi Comprehens Canc Ctr, Washington, DC 20057 USAGeorgetown Univ, Dept Oncol, Washington, DC 20057 USA
Johnson, Peter
[1
,2
]
Mueller, Susette
论文数: 0引用数: 0
h-index: 0
机构:
Georgetown Univ, Dept Oncol, Washington, DC 20057 USA
Georgetown Univ, Lombardi Comprehens Canc Ctr, Washington, DC 20057 USAGeorgetown Univ, Dept Oncol, Washington, DC 20057 USA
Mueller, Susette
[1
,2
]
Hoppe, Philipp S.
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机构:
German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Munich, GermanyGeorgetown Univ, Dept Oncol, Washington, DC 20057 USA
Hoppe, Philipp S.
[3
]
Schroeder, Timm
论文数: 0引用数: 0
h-index: 0
机构:
German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Munich, GermanyGeorgetown Univ, Dept Oncol, Washington, DC 20057 USA
Schroeder, Timm
[3
]
Furth, Priscilla A.
论文数: 0引用数: 0
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机构:
Georgetown Univ, Dept Oncol, Washington, DC 20057 USA
Georgetown Univ, Lombardi Comprehens Canc Ctr, Washington, DC 20057 USA
Georgetown Univ, Dept Med, Washington, DC 20057 USA
Dankook Univ, Dept Nanobiomed Sci, Yongin, South Korea
Dankook Univ, WCU Res Ctr Nanobiomed Sci, Yongin, South KoreaGeorgetown Univ, Dept Oncol, Washington, DC 20057 USA
Furth, Priscilla A.
[1
,2
,4
,5
,6
]
机构:
[1] Georgetown Univ, Dept Oncol, Washington, DC 20057 USA
[2] Georgetown Univ, Lombardi Comprehens Canc Ctr, Washington, DC 20057 USA
[3] German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Munich, Germany
[4] Georgetown Univ, Dept Med, Washington, DC 20057 USA
[5] Dankook Univ, Dept Nanobiomed Sci, Yongin, South Korea
[6] Dankook Univ, WCU Res Ctr Nanobiomed Sci, Yongin, South Korea
来源:
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
|
2013年
/
72期
Time-lapse imaging can be used to compare behavior of cultured primary preneoplastic mammary epithelial cells derived from different genetically engineered mouse models of breast cancer. For example, time between cell divisions (cell lifetimes), apoptotic cell numbers, evolution of morphological changes, and mechanism of colony formation can be quantified and compared in cells carrying specific genetic lesions. Primary mammary epithelial cell cultures are generated from mammary glands without palpable tumor. Glands are carefully resected with clear separation from adjacent muscle, lymph nodes are removed, and single-cell suspensions of enriched mammary epithelial cells are generated by mincing mammary tissue followed by enzymatic dissociation and filtration. Single-cell suspensions are plated and placed directly under a microscope within an incubator chamber for live-cell imaging. Sixteen 650 mu m x 700 mu m fields in a 4x4 configuration from each well of a 6-well plate are imaged every 15 min for 5 days. Time-lapse images are examined directly to measure cellular behaviors that can include mechanism and frequency of cell colony formation within the first 24 hr of plating the cells (aggregation versus cell proliferation), incidence of apoptosis, and phasing of morphological changes. Single-cell tracking is used to generate cell fate maps for measurement of individual cell lifetimes and investigation of cell division patterns. Quantitative data are statistically analyzed to assess for significant differences in behavior correlated with specific genetic lesions.
机构:
Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Canc Biol Program, Boston, MA 02215 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Burga, Laura N.
Tung, Nadine M.
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h-index: 0
机构:
Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Tung, Nadine M.
Troyan, Susan L.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Dept Surg, Boston, MA 02215 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Troyan, Susan L.
Bostina, Mihnea
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02215 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Bostina, Mihnea
Konstantinopoulos, Panagiotis A.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Konstantinopoulos, Panagiotis A.
Fountzilas, Helena
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Fountzilas, Helena
Spentzos, Dimitrios
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Spentzos, Dimitrios
Miron, Alexander
论文数: 0引用数: 0
h-index: 0
机构:
Dana Farber Canc Inst, Dept Canc Biol, Boston, MA 02115 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Miron, Alexander
Yassin, Yosuf A.
论文数: 0引用数: 0
h-index: 0
机构:
Dana Farber Canc Inst, Dept Canc Biol, Boston, MA 02115 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Yassin, Yosuf A.
Lee, Bernard T.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Dept Surg, Boston, MA 02215 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Lee, Bernard T.
Wulf, Gerburg M.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Canc Biol Program, Boston, MA 02215 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
机构:
German Res Ctr Environm Hlth, Res Unit Stem Cell Dynam, Helmholtz Ctr Munich, Neuherberg, GermanyGerman Res Ctr Environm Hlth, Res Unit Stem Cell Dynam, Helmholtz Ctr Munich, Neuherberg, Germany
Kokkaliaris, Konstantinos D.
Loeffler, Dirk
论文数: 0引用数: 0
h-index: 0
机构:
German Res Ctr Environm Hlth, Res Unit Stem Cell Dynam, Helmholtz Ctr Munich, Neuherberg, GermanyGerman Res Ctr Environm Hlth, Res Unit Stem Cell Dynam, Helmholtz Ctr Munich, Neuherberg, Germany
Loeffler, Dirk
Schroeder, Timm
论文数: 0引用数: 0
h-index: 0
机构:
German Res Ctr Environm Hlth, Res Unit Stem Cell Dynam, Helmholtz Ctr Munich, Neuherberg, GermanyGerman Res Ctr Environm Hlth, Res Unit Stem Cell Dynam, Helmholtz Ctr Munich, Neuherberg, Germany
机构:
German Res Ctr Environm Hlth, Helmholtz Zentrum Muenchen, Neuherberg, GermanyGerman Res Ctr Environm Hlth, Helmholtz Zentrum Muenchen, Neuherberg, Germany
Rieger, Michael A.
Schroeder, Timm
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h-index: 0
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German Res Ctr Environm Hlth, Helmholtz Zentrum Muenchen, Neuherberg, GermanyGerman Res Ctr Environm Hlth, Helmholtz Zentrum Muenchen, Neuherberg, Germany
机构:
Helmholtz Zentrum Munchen, German Res Ctr Environm Hlth, Inst Stem Cell Res, D-85764 Neuherberg, GermanyHelmholtz Zentrum Munchen, German Res Ctr Environm Hlth, Inst Stem Cell Res, D-85764 Neuherberg, Germany
机构:
Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Canc Biol Program, Boston, MA 02215 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Burga, Laura N.
Tung, Nadine M.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Tung, Nadine M.
Troyan, Susan L.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Dept Surg, Boston, MA 02215 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Troyan, Susan L.
Bostina, Mihnea
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02215 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Bostina, Mihnea
Konstantinopoulos, Panagiotis A.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Konstantinopoulos, Panagiotis A.
Fountzilas, Helena
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Fountzilas, Helena
Spentzos, Dimitrios
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Spentzos, Dimitrios
Miron, Alexander
论文数: 0引用数: 0
h-index: 0
机构:
Dana Farber Canc Inst, Dept Canc Biol, Boston, MA 02115 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Miron, Alexander
Yassin, Yosuf A.
论文数: 0引用数: 0
h-index: 0
机构:
Dana Farber Canc Inst, Dept Canc Biol, Boston, MA 02115 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Yassin, Yosuf A.
Lee, Bernard T.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Dept Surg, Boston, MA 02215 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Lee, Bernard T.
Wulf, Gerburg M.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Canc Biol Program, Boston, MA 02215 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Hematol Oncol, Boston, MA 02215 USA
机构:
German Res Ctr Environm Hlth, Res Unit Stem Cell Dynam, Helmholtz Ctr Munich, Neuherberg, GermanyGerman Res Ctr Environm Hlth, Res Unit Stem Cell Dynam, Helmholtz Ctr Munich, Neuherberg, Germany
Kokkaliaris, Konstantinos D.
Loeffler, Dirk
论文数: 0引用数: 0
h-index: 0
机构:
German Res Ctr Environm Hlth, Res Unit Stem Cell Dynam, Helmholtz Ctr Munich, Neuherberg, GermanyGerman Res Ctr Environm Hlth, Res Unit Stem Cell Dynam, Helmholtz Ctr Munich, Neuherberg, Germany
Loeffler, Dirk
Schroeder, Timm
论文数: 0引用数: 0
h-index: 0
机构:
German Res Ctr Environm Hlth, Res Unit Stem Cell Dynam, Helmholtz Ctr Munich, Neuherberg, GermanyGerman Res Ctr Environm Hlth, Res Unit Stem Cell Dynam, Helmholtz Ctr Munich, Neuherberg, Germany
机构:
German Res Ctr Environm Hlth, Helmholtz Zentrum Muenchen, Neuherberg, GermanyGerman Res Ctr Environm Hlth, Helmholtz Zentrum Muenchen, Neuherberg, Germany
Rieger, Michael A.
Schroeder, Timm
论文数: 0引用数: 0
h-index: 0
机构:
German Res Ctr Environm Hlth, Helmholtz Zentrum Muenchen, Neuherberg, GermanyGerman Res Ctr Environm Hlth, Helmholtz Zentrum Muenchen, Neuherberg, Germany
机构:
Helmholtz Zentrum Munchen, German Res Ctr Environm Hlth, Inst Stem Cell Res, D-85764 Neuherberg, GermanyHelmholtz Zentrum Munchen, German Res Ctr Environm Hlth, Inst Stem Cell Res, D-85764 Neuherberg, Germany