Environmentally Friendly Extraction of Bioactive Compounds from Rosa canina L. fruits Using Deep Eutectic Solvent (DES) as Green Extraction Media

In this study, the green extraction of bioactive compounds from Rosehip (Rosa canina L.) fruits and their antioxidant activity were investigated. An ultrasound-assisted extraction combined with deep eutectic solvents (DES) was used for this purpose. Deep eutectic solvents based on citric acid were specially designed. Namely, hydrogen bond donor (HBD) such as glycerol and ethylene glycol as well as hydrogen bond acceptor (HBA) like citric acid were used. After choosing the best option of DES, for extraction of the bioactive ingredients, optimal extraction conditions of the ultrasonic-assisted extraction have been optimized through Box-Behnken design of response surface methodology (RSM). Total phenolics content (TPC), total anthocyanins content (TAA), and antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) have been found as 103.37 mg GAE/g DW in DES2, 92.23 mg GAE/g DW in DES1, 3.25mg C3G/100g-DW in DES2, 1.31 mg C3G/100g-DW in DES1, and 101.85% inhibition in DES2, 94.32%. The results of this study showed that this method is a competitive sustainable, green, and effective extraction of bioactive compounds from Rosehip (Rosa canina L.) fruits.