Identification of bifidobacteria isolated from Asian elephant (Elephas maximus)

被引：6

作者：

Bunesova, Vera ^{[1
]}

Vlkova, Eva ^{[1
]}

Rada, Vojtech ^{[1
]}

Killer, Jiri ^{[1
,3
]}

Kmet, Vladimir ^{[2
]}

机构：

[1] Czech Univ Life Sci Prague, Fac Agrobiol Food & Nat Resources, Dept Microbiol Nutr & Dietet, Prague 16521 6, Suchdol, Czech Republic

[2] Slovak Acad Sci, Inst Anim Physiol, Kosice 04001, Slovakia

[3] Acad Sci Czech Republic, Inst Anim Physiol & Genet, Prague 14200 4, Krc, Czech Republic

来源：

JOURNAL OF BIOSCIENCES | 2013年 / 38卷 / 02期

关键词：

16S rRNA genes sequencing; bifidobacteria; Elephas maximus; herbivores; identification; SP NOV; FECES; STRAINS; TRACT; GENE; PCR;

D O I：

10.1007/s12038-013-9322-y

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Bifidobacteria are considered as one of the key genera in intestinal tracts of animals, and their species composition vary depending on the host. The aim of this study was to identify faecal bifidobacteria from Asian elephants (Elephas maximus), housed in Zoological gardens (Ostrava, Czech Republic). Using culturing, bifidobacteria were found in counts 7.60 +/- 0.56 log CFU/g. Twenty-six pure strains were isolated from faeces of Asian elephant. The isolates were clustered into two groups according to fingerprinting profiles and fermentation characteristic. Bacteria were identified by a combination of MALDI-TOF MS, PCR methods and sequencing as B. boum (12 isolates) and B. adolescentis (14 isolates). Elephant strains showed different fingerprinting profiles than type and collection strains. Since these two species are frequently isolated from gastrointestinal tract of herbivores, they seem to be typical of animals fed plant diets.

引用

页码：239 / 243

页数：5

共 20 条

[1] Fatal enterocolitis in Asian elephants (Elephas maximus) caused by Clostridium difficile [J].

Bojesen, Anders Miki ;

Olsen, Katharina E. P. ;

Bertelsen, Mads F. .

VETERINARY MICROBIOLOGY, 2006, 116 (04) :329-335

[2] Multiplex PCR with 16S rRNA gene-targeted primers of Bifidobacterium spp. to identify sources of fecal pollution [J].

Bonjoch, X ;

Ballesé, E ;

Blanch, AR .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (05) :3171-3175

[3] Identification of Bifidobacterium strains from faeces of lambs [J].

Bunesova, Vera ;

Vlkova, Eva ;

Killer, Jiri ;

Rada, Vojtech ;

Rockova, Sarka .

SMALL RUMINANT RESEARCH, 2012, 105 (1-3) :355-360

[4] Probiotics in animal nutrition and health [J].

Chaucheyras-Durand, F. ;

Durand, H. .

BENEFICIAL MICROBES, 2010, 1 (01) :3-9

[5] Bifidobacterium reuteri sp nov., Bifidobacterium callitrichos sp nov., Bifidobacterium saguini sp nov., Bifidobacterium stellenboschense sp nov and Bifidobacterium biavatii sp nov isolated from faeces of common marmoset (Callithrix jacchus) and red-handed tamarin (Saguinus midas) [J].

Endo, Akihito ;

Futagawa-Endo, Yuka ;

Schumann, Peter ;

Pukall, Ruediger ;

Dicks, Leon M. T. .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 2012, 35 (02) :92-97

[6] Diversity of Lactobacillus and Bifidobacterium in feces of herbivores, omnivores and carnivores [J].

Endo, Akihito ;

Futagawa-Endo, Yuka ;

Dicks, Leon M. T. .

ANAEROBE, 2010, 16 (06) :590-596

[7] Applicability of rep-PCR fingerprinting for identification of Lactobacillus species [J].

Gevers, D ;

Huys, G ;

Swings, J .

FEMS MICROBIOLOGY LETTERS, 2001, 205 (01) :31-36

[8] Bombiscardovia coagulans gen. nov., sp nov., a new member of the family Bifidobacteriaceae isolated from the digestive tract of bumblebees [J].

Killer, J. ;

Kopecny, J. ;

Mrazek, J. ;

Havlik, J. ;

Koppova, I. ;

Benada, O. ;

Rada, V. ;

Kofronova, O. .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 2010, 33 (07) :359-366

[9] Antimicrobial susceptibility of microflora from ovine cheese [J].

Kmet', V. ;

Drugdova, Z. .

FOLIA MICROBIOLOGICA, 2012, 57 (04) :291-293

[10] Bifidobacteria in feces and environmental waters [J].

Lamendella, Regina ;

Domingo, Jorge W. Santo ;

Kelty, Catherine ;

Oerther, Daniel B. .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (03) :575-584

← 1 2 →