Cell-free protein synthesis: Applications come of age

被引:502
作者
Carlson, Erik D. [1 ,2 ]
Gan, Rui [1 ,2 ]
Hodgman, C. Eric [1 ,2 ]
Jewett, Michael C. [1 ,2 ]
机构
[1] Northwestern Univ, Dept Chem & Biol Engn, Evanston, IL 60208 USA
[2] Northwestern Univ, Chem Life Proc Inst, Evanston, IL 60208 USA
基金
美国国家卫生研究院; 美国国家科学基金会;
关键词
Cell-free protein synthesis; In vitro translation; Recombinant DNA protein production; E. coli extract; Wheat germ extract; Personalized medicines; High-throughput; Synthetic biology; Cell-free biology; INTEGRAL MEMBRANE-PROTEINS; VITRO TRANSLATION SYSTEM; AMINO-ACID STABILIZATION; IN-VITRO; FREE EXPRESSION; HIGH-THROUGHPUT; DIRECTED EVOLUTION; RIBOSOME-DISPLAY; RAPID EXPRESSION; FUSION PROTEINS;
D O I
10.1016/j.biotechadv.2011.09.016
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Cell-free protein synthesis has emerged as a powerful technology platform to help satisfy the growing demand for simple and efficient protein production. While used for decades as a foundational research tool for understanding transcription and translation, recent advances have made possible cost-effective microscale to manufacturing scale synthesis of complex proteins. Protein yields exceed grams protein produced per liter reaction volume, batch reactions last for multiple hours, costs have been reduced orders of magnitude. and reaction scale has reached the 100-liter milestone. These advances have inspired new applications in the synthesis of protein libraries for functional genomics and structural biology, the production of personalized medicines, and the expression of virus-like particles. among others. In the coming years. cell-free protein synthesis promises new industrial processes where short protein production timelines are crucial as well as innovative approaches to a wide range of applications. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码:1185 / 1194
页数:10
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