Cryopreservation by encapsulation-dehydration of plumules of coconut (Cocos nucifera L.)

被引:2
作者
N'Nan, Oulo [1 ,4 ]
Hocher, Valerie [1 ]
Verdeil, Jean-Luc [2 ]
Konan, Jean-Louis [3 ]
Ballo, Koffi [3 ]
Mondeil, Fanja [4 ]
Malaurie, Bernard [1 ]
机构
[1] IRD, F-34394 Montpellier 5, France
[2] CIRAD, F-34398 Montpellier 5, France
[3] Stn Marc Delorme CNRA, Abidjan 07, Cote Ivoire
[4] Univ Abidjan Cocody, Genet Lab, UFR Biosci, Abidjan 22, Cote Ivoire
关键词
Cocos nucifera L; cryopreservation; encapsulation-dehydration; histology; plumule; sucrose;
D O I
暂无
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
This study describes the use of an encapsulation-dehydration cryopreservation technique on coconut plumules (apical dome with three or four leaf primordia) excised from embryos. In order to establish a reliable cryopreservation process for plumules, several different key factors were tested: pretreatment duration, sugar concentration, dehydration period and freezing. In parallel, histological studies were performed to describe the structural changes of tissues and plumule cells subjected to dehydration and freezing. A good survival level of around 60% was obtained. However, after 8 months culture regrowth, this level decreased to a maximum of 20% which was achieved using sucrose treatment. In this paper we report for the first time the regeneration of leafy shoots from coconut plumules after cryopreservation.
引用
收藏
页码:339 / 350
页数:12
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