Arabidopsis microRNA expression regulation in a wide range of abiotic stress responses

被引：144

作者：

Barciszewska-Pacak, Maria ^{[1
]}

Milanowska, Kaja ^{[1
]}

Knop, Katarzyna ^{[1
]}

Bielewicz, Dawid ^{[1
]}

Nuc, Przemyslaw ^{[1
]}

Plewka, Patrycja ^{[1
]}

Pacak, Andrzej M. ^{[1
]}

Vazquez, Franck ^{[2
]}

Karlowski, Wojciech ^{[3
]}

Jarmolowki, Artur ^{[1
]}

Szweykowska-Kulinska, Zofia ^{[1
]}

机构：

[1] Adam Mickiewicz Univ, Inst Mol Biol & Biotechnol, Fac Biol, Dept Gene Express, PL-61614 Poznan, Poland

[2] Univ Basel, Inst Bot, Swiss Plant Sci Web, Zurich Basel Plant Sci Ctr, CH-4056 Basel, Switzerland

[3] Adam Mickiewicz Univ, Inst Mol Biol & Biotechnol, Fac Biol, Dept Computat Biol, PL-61614 Poznan, Poland

来源：

FRONTIERS IN PLANT SCIENCE | 2015年 / 6卷

关键词：

miRNA; pri-miRNA; abiotic stress; gene expression; MICROARRAY-BASED ANALYSIS; PRI-MIRNA; TRANSCRIPTION FACTORS; OXIDATIVE STRESS; GENE-EXPRESSION; DOWN-REGULATION; MESSENGER-RNA; BIOGENESIS; HYL1; IDENTIFICATION;

D O I：

10.3389/fpls.2015.00410

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Arabidopsis microRNA expression regulation was studied in a wide array of abiotic stresses such as drought, heat, salinity, copper excess/deficiency, cadmium excess, and sulfur deficiency. A home-built RT-qPCR mirEX platform for the amplification of 289 Arabidopsis microRNA transcripts was used to study their response to abiotic stresses. Small RNA sequencing, Northern hybridization, and TaqMan(R) microRNA assays were performed to study the abundance of mature microRNAs. A broad response on the level of primary miRNAs (pri-miRNAs) was observed. However, stress response at the level of mature microRNAs was rather confined. The data presented show that in most instances, the level of a particular mature miRNA could not be predicted based on the level of its pri-miRNA. This points to an essential role of posttranscriptional regulation of microRNA expression. New Arabidopsis microRNAs responsive to abiotic stresses were discovered. Four microRNAs: miR319a/b, miR319b.2, and miR400 have been found to be responsive to several abiotic stresses and thus can be regarded as general stress-responsive microRNA species.

引用

页数：14

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[11] Identification of chilling stress-responsive tomato microRNAs and their target genes by high-throughput sequencing and degradome analysis [J].