Characterization of temperature dependent modifier effects in SFC using linear solvation energy relationships

被引:35
作者
Blackwell, JA
Stringham, RW
机构
[1] Dupont Merck Pharmaceutical Company, Chem. Proc. Research and Development, Chambers Works, PRF-1 (S-1), Deepwater
关键词
supercritical fluid chromatography; packed columns; selectivity relationships; hydrogen bonding; linear solvation energy relationships;
D O I
10.1007/BF02496323
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Linear solvation energy relationships (LSERs) are used to probe the changes in mobile and stationary phase properties of a carbon dioxide-based mobile phase and a polymeric stationary phase under near-critical conditions. Four mobile phase modifiers are compared with respect to dipolarity/polarizability, hydrogen bond donating and accepting ability, and other intermolecular interactions as a function of temperature. As temperature nears the mixture critical point, the differences in these properties between the mobile and stationary phases change to reflect the growing heterogeneity in mobile phase component distribution at the chromatographic interface. The stationary phase loses many of its original characteristics and takes on characteristics typical of the mobile phase modifier due to preferential adsorption of the modifier at the surface of the stationary phase.
引用
收藏
页码:301 / 308
页数:8
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