Impact of assisted reproduction, infertility, sex and paternal factors on the placental DNA methylome

被引：63

作者：

Choufani, Sanaa ^{[1
]}

Turinsky, Andrei L. ^{[1
,2
]}

Melamed, Nir ^{[3
]}

Greenblatt, Ellen ^{[4
]}

Brudno, Michael ^{[1
,2
,5
]}

Berard, Anick ^{[6
,7
,18
]}

Fraser, William D. ^{[8
,9
,17
]}

Weksberg, Rosanna ^{[1
,10
,11
]}

Trasler, Jacquetta ^{[12
,13
,14
,15
,22
]}

Monnier, Patricia ^{[15
,16
,22
]}

Fraser, William D. ^{[8
,9
,17
]}

Audibert, Francois ^{[18
]}

Dubois, Lise ^{[19
]}

Julien, Pierre ^{[20
]}

Luo, Zhong-Cheng ^{[21
]}

Michaud, Jacques ^{[18
]}

Moutquin, Jean-Marie ^{[17
]}

Muckle, Gina ^{[20
]}

Seguin, Jean ^{[18
]}

Trasler, Jacquetta ^{[12
,13
,14
,15
,22
]}

Tremblay, Richard Ernest ^{[18
]}

Abenhaim, Haim ^{[22
]}

Bedard, Marie-Josee ^{[18
]}

Berard, Anick ^{[6
,7
,18
]}

Bujold, Emmanuel ^{[20
]}

Gagnon, Robert ^{[22
]}

Girard, Isabelle ^{[22
]}

Kibar, Zoha ^{[18
]}

Marc, Isabelle ^{[20
]}

Monnier, Patricia ^{[15
,16
,22
]}

Simard, Marie-Noelle ^{[18
]}

Pasquier, Jean-Charles ^{[17
]}

Welt, Michel ^{[18
]}

机构：

[1] Hosp Sick Children, Genet & Genome Biol Program, Toronto, ON, Canada

[2] Hosp Sick Children, Ctr Computat Med, Toronto, ON, Canada

[3] Sunnybrook Hlth Sci Ctr, Dept Obstet & Gynaecol, Div Maternal Fetal Med, Toronto, ON, Canada

[4] Mt Sinai Hosp, Mt Sinai Ctr Fertil & Reprod Hlth, Toronto, ON, Canada

[5] Univ Toronto, Dept Comp Sci, Toronto, ON, Canada

[6] Univ Montreal, CHU St Justine, Res Ctr, Res Unit Medicat & Pregnancy, Montreal, PQ, Canada

[7] Univ Montreal, Fac Pharm, Montreal, PQ, Canada

[8] Univ Sherbrooke, Dept Obstet & Gynecol, Sherbrooke, PQ, Canada

[9] Ctr Rech CHUS, Sherbrooke, PQ, Canada

[10] Hosp Sick Children, Div Clin & Metab Genet, Toronto, ON, Canada

[11] Univ Toronto, Dept Pediat & Inst Med Sci, Toronto, ON, Canada

[12] McGill Univ, Montreal Childrens Hosp, Dept Pediat, Hlth Ctr, Quebec City, PQ, Canada

[13] McGill Univ, Montreal Childrens Hosp, Dept Human Genet, Hlth Ctr, Quebec City, PQ, Canada

[14] McGill Univ, Montreal Childrens Hosp, Dept Pharmacol & Therapeut, Hlth Ctr, Quebec City, PQ, Canada

[15] McGill Univ, Hlth Ctr, Res Inst, 1001 Boul Decarie,Block E-EM03211, Quebec City, PQ H4A 3J1, Canada

[16] McGill Univ, Royal Victoria Hosp, Dept Obstet & Gynecol, MUHC Reprod Ctr,Hlth Ctr, Quebec City, PQ, Canada

[17] Univ Sherbrooke, Sherbrooke, PQ, Canada

[18] Univ Montreal, Montreal, PQ, Canada

[19] Univ Ottawa, Ottawa, ON, Canada

[20] Univ Laval, Laval, PQ, Canada

[21] Univ Toronto, Toronto, ON, Canada

[22] McGill Univ, Quebec City, PQ, Canada

来源：

HUMAN MOLECULAR GENETICS | 2019年 / 28卷 / 03期

基金：

加拿大健康研究院; 加拿大自然科学与工程研究理事会;

关键词：

BECKWITH-WIEDEMANN-SYNDROME; IN-VITRO FERTILIZATION; IMPRINTED GENES; METHYLATION LEVELS; GENOME-WIDE; SPERM; TECHNOLOGY; RATIO; RISK; EXPRESSION;

D O I：

10.1093/hmg/ddy321

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Children conceived using Assisted Reproductive Technologies (ART) have a higher incidence of growth and birth defects, attributable in part to epigenetic perturbations. Both ART and germline defects associated with parental infertility could interfere with epigenetic reprogramming events in germ cells or early embryos. Mouse models indicate that the placenta is more susceptible to the induction of epigenetic abnormalities than the embryo, and thus the placental methylome may provide a sensitive indicator of 'at risk' conceptuses. Our goal was to use genome-wide profiling to examine the extent of epigenetic abnormalities in matched placentas from an ART/infertility group and control singleton pregnancies (n = 44/group) from a human prospective longitudinal birth cohort, the Design, Develop, Discover (3D) Study. Principal component analysis revealed a group of ART outliers. The ART outlier group was enriched for females and a subset of placentas showing loss of methylation of several imprinted genes including GNAS, SGCE, KCNQT1OT1 and BLCAP/NNAT. Within the ART group, placentas from pregnancies conceived with in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) showed distinct epigenetic profiles as compared to those conceived with less invasive procedures (ovulation induction, intrauterine insemination). Male factor infertility and paternal age further differentiated the IVF/ICSI group, suggesting an interaction of infertility and techniques in perturbing the placental epigenome. Together, the results suggest that the human placenta is sensitive to the induction of epigenetic defects by ART and/or infertility, and we stress the importance of considering both sex and paternal factors and that some but not all ART conceptuses will be susceptible.

引用

页码：372 / 385

页数：14

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