Genetic Transformation in Citrus

被引:30
作者
Donmez, Dicle [1 ]
Simsek, Ozhan [2 ]
Izgu, Tolga [3 ]
Kacar, Yildiz Aka [1 ,2 ]
Mendi, Yesim Yalcin [1 ,2 ]
机构
[1] Cukurova Univ, Inst Appl & Nat Sci, Dept Biotechnol, TR-01330 Adana, Turkey
[2] Cukurova Univ, Fac Agr, Dept Hort, TR-01330 Adana, Turkey
[3] Ege Univ, Fac Agr, Dept Hort, TR-35100 Izmir, Turkey
关键词
AGROBACTERIUM-MEDIATED TRANSFORMATION; TRIFOLIATA L. RAF; COAT PROTEIN GENE; VALENCIA SWEET ORANGE; GREEN FLUORESCENT PROTEIN; PHLOEM-SPECIFIC PROMOTERS; RNA-POLYMERASE GENES; TRISTEZA-VIRUS CTV; TRANSGENIC PLANTS; PARADISI MACF;
D O I
10.1155/2013/491207
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Citrus is one of the world's important fruit crops. Recently, citrus molecular genetics and biotechnology work have been accelerated in the world. Genetic transformation, a biotechnological tool, allows the release of improved cultivars with desirable characteristics in a shorter period of time and therefore may be useful in citrus breeding programs. Citrus transformation has now been achieved in a number of laboratories by various methods. Agrobacterium tumefaciens is used mainly in citrus transformation studies. Particle bombardment, electroporation, A. rhizogenes, and a new method called RNA interference are used in citrus transformation studies in addition to A. tumefaciens. In this review, we illustrate how different gene transformation methods can be employed in different citrus species.
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页数:8
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