Effects of Phytoestrogen Extracts Isolated from Pumpkin Seeds on Estradiol Production and ER/PR Expression in Breast Cancer and Trophoblast Tumor Cells

被引：22
|
作者：
Richter, Dagmar [1 ]
Abarzua, Sibylle [2 ]
Chrobak, Mareike [3 ]
Vrekoussis, Thomas [4 ]
Weissenbacher, Tobias [4 ]
Kuhn, Christina [4 ]
Schulze, Sandra [4 ]
Kupka, Markus S. [4 ]
Friese, Klaus [4 ]
Briese, Volker [1 ]
Piechulla, Birgit [2 ]
Makrigiannakis, Antonis [5 ]
Jeschke, Udo [4 ]
Dian, Darius [4 ]
机构：
[1] Univ Rostock, Dept Obstet & Gynaecol, D-18055 Rostock, Germany
[2] Univ Rostock, Dept Biol Sci, D-18055 Rostock, Germany
[3] Bernhard Nocht Inst Trop Med, D-20359 Hamburg, Germany
[4] Univ Munich, Dept Obstet & Gynaecol, D-80337 Munich, Germany
[5] Univ Crete, Sch Med, Dept Obstet & Gynecol, Athens, Greece
来源：
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL | 2013年 / 65卷 / 05期
关键词：
PHYTO-ESTROGENS; GROWTH; PROLIFERATION; AROMATASE; GENISTEIN; FLAXSEED; SOY; PROGESTERONE; METABOLISM; LIGNANS;
D O I：
10.1080/01635581.2013.797000
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Phytoestrogens have a controversial effect on hormone-dependent tumours. Herein, we investigated the effect of the pumpkin seed extract (PSE) on estradiol production and estrogen receptor (ER)-/ER-/progesterone receptor (PR) status on MCF7, Jeg3, and BeWo cells. The PSE was prepared and analyzed by mass spectrometry. MCF7, Jeg3, and BeWo cells were incubated with various concentrations of PSE. Untreated cells served as controls. Supernatants were tested for estradiol production with an ELISA method. Furthermore, the effect of the PSE on ER-/ER-/PR expression was assessed by immunocytochemistry. The PSE was found to contain both lignans and flavones. Estradiol production was elevated in MCF7, BeWo, and Jeg3 cells in a concentration-dependent manner. In MCF7 cells, a significant ER- downregulation and a significant PR upregulation were observed. The above results after properly designed animal studies could highlight a potential role of pumpkin seed's lignans in breast cancer prevention and/or treatment.
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收藏
页码：739 / 745
页数：7
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