Expression stability of marker gene npt II in transgenic plants Nicotiana tobacum L. with single T-DNA insertion

被引:1
|
作者
Novoselia, V [1 ]
Deineko, EV [1 ]
Filipenko, EA [1 ]
Shumnyi, VK [1 ]
机构
[1] Russian Acad Sci, Inst Cytol & Genet, Siberian Branch, Novosibirsk, Russia
关键词
D O I
10.1080/13102818.2001.10819121
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Expression stability of marker gene nptII in transgenic tobacco plants, Nicotiana tobacum L, line SRI with single transgenic insertion was being investigated. Stable expression of gene nptII has been shown in the first (T-1) and second (T-2) generations from the selfing of plants under study and in F-1 when crossing plants T-1 homozygous on the transgene. Deviations from the theoretically expected segregation were registered in F-2 at 18%.
引用
收藏
页码:3 / 7
页数:5
相关论文
共 50 条
  • [31] PHOTOSYNTHESIS AND WATER RELATIONS IN TRANSGENIC TOBACCO PLANTS WITH T-DNA CARRYING GENE 4 FOR CYTOKININ SYNTHESIS
    CATSKY, J
    POSPISILOVA, J
    MACHACKOVA, I
    WILHELMOVA, N
    SESTAK, Z
    BIOLOGIA PLANTARUM, 1993, 35 (03) : 393 - 399
  • [32] STABILITY OF A FOREIGN GENE IN TRANSGENIC NICOTIANA-TABACUM-L PLANTS DURING A CYCLE OF DEDIFFERENTIATION DIFFERENTIATION
    BELLINI, C
    GIORDANI, C
    LUPOTTO, E
    LOCATELLI, F
    CUZZONI, E
    AVOGADRO, E
    CASTIGLIONE, S
    SALA, F
    PLANT SCIENCE, 1992, 82 (02) : 193 - 200
  • [33] Gene stability in transgenic aspen-populus -: III.: T-DNA repeats influence transgene expression differentially among different transgenic lines
    Fladung, M
    Kumar, S
    PLANT BIOLOGY, 2002, 4 (03) : 329 - 338
  • [34] Science Letters:Expression of a begomoviral DNAβ gene in transgenic Nicotiana plants induced abnormal cell division
    崔晓峰
    李云琴
    胡东维
    周雪平
    Journal of Zhejiang University Science, 2005, (02) : 7 - 10
  • [35] An optimized double T-DNA binary vector system for improved production of marker-free transgenic tobacco plants
    Leng, Chunxu
    Sun, Bing
    Liu, Zheming
    Zhang, Lei
    Wei, Xiaoli
    Zhou, Yun
    Meng, Ying
    Lai, Yongcai
    Dai, Yan
    Zhu, Zhen
    BIOTECHNOLOGY LETTERS, 2020, 42 (04) : 641 - 655
  • [36] An optimized double T-DNA binary vector system for improved production of marker-free transgenic tobacco plants
    Chunxu Leng
    Bing Sun
    Zheming Liu
    Lei Zhang
    Xiaoli Wei
    Yun Zhou
    Ying Meng
    Yongcai Lai
    Yan Dai
    Zhen Zhu
    Biotechnology Letters, 2020, 42 : 641 - 655
  • [37] Cadmium resistance in transgenic tobacco plants expressing the Nicotiana glutinosa L. metallothionein-like gene
    Suh, MC
    Choi, D
    Liu, JR
    MOLECULES AND CELLS, 1998, 8 (06) : 678 - 684
  • [38] Complete sequence of the binary vector pBI121 and its application in cloning T-DNA insertion from transgenic plants
    Po-Yen Chen
    Chen-Kuen Wang
    Shaw-Ching Soong
    Kin-Ying To
    Molecular Breeding, 2003, 11 : 287 - 293
  • [39] Instability of NPTII gene expression in the progeny of transgenic tobacco plants (Nicotiana tabacum L and N-plumbaginifolia L)
    Deineko, E
    Zagorskaya, A
    Filipenko, E
    Filipenko, M
    Novoselya, T
    Kochetov, A
    Komarova, M
    Shumnyi, V
    BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 1996, 10 (04): : 89 - 92
  • [40] Complete sequence of the binary vector pBI121 and its application in cloning T-DNA insertion from transgenic plants
    Chen, PY
    Wang, CK
    Soong, SC
    To, KY
    MOLECULAR BREEDING, 2003, 11 (04) : 287 - 293