Generating Embryonic Stem Cells from the Inbred Mouse Strain DBA/2J, a Model of Glaucoma and Other Complex Diseases

被引：6

作者：

Reinholdt, Laura G. ^{[1
]}

Howell, Gareth R. ^{[1
,2
]}

Czechanski, Anne M. ^{[1
]}

Macalinao, Danilo G. ^{[1
]}

MacNicoll, Katharine H. ^{[1
]}

Lin, Chyuan-Sheng ^{[3
]}

Donahue, Leah Rae ^{[1
]}

John, Simon W. M. ^{[1
,2
,4
]}

机构：

[1] Jackson Lab, Bar Harbor, ME 04609 USA

[2] Howard Hughes Med Inst, Bar Harbor, ME USA

[3] Columbia Univ, Dept Pathol & Cell Biol, Irving Canc Res Ctr, New York, NY USA

[4] Tufts Univ Med, Dept Ophthalmol, Boston, MA USA

来源：

PLOS ONE | 2012年 / 7卷 / 11期

关键词：

OPTIC-NERVE DEGENERATION; HOST RESPONSE; GROUND-STATE; MICE; NEURODEGENERATION; INFECTION; C57BL/6J; GENETICS;

D O I：

10.1371/journal.pone.0050081

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mouse embryonic stem (ES) cells are derived from the inner cell mass of blastocyst stage embryos and are used primarily for the creation of genetically engineered strains through gene targeting. While some inbred strains of mice are permissive to the derivation of embryonic stem cell lines and are therefore easily engineered, others are nonpermissive or recalcitrant. Genetic engineering of recalcitrant strain backgrounds requires gene targeting in a permissive background followed by extensive backcrossing of the engineered allele into the desired strain background. The inbred mouse strain DBA/2J is a recalcitrant strain that is used as a model of many human diseases, including glaucoma, deafness and schizophrenia. Here, we describe the generation of germ-line competent ES cell lines derived from DBA/2J mice. We also demonstrate the utility of DBA/2J ES cells with the creation of conditional knockout allele for Endothelin-2 (Edn2) directly on the DBA/2J strain background.

引用

页数：8

共 37 条

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