Development and characterization of 22 polymorphic microsatellite markers for the balloon flower Platycodon grandiflorum (Campanulaceae)

被引：5

作者：

Song, J. Y. ^{[1
]}

Lee, G. -A. ^{[1
]}

Yoon, M. -S. ^{[1
]}

Ma, K. -H. ^{[1
]}

Choi, Y. -M. ^{[1
]}

Lee, J. -R. ^{[1
]}

Park, H. -J. ^{[1
]}

Lee, M. -C. ^{[1
]}

机构：

[1] Natl Acad Agr Sci, Rural Dev Adm, Natl Agrobiodivers Ctr, Suwon, South Korea

来源：

GENETICS AND MOLECULAR RESEARCH | 2012年 / 11卷 / 03期

关键词：

Platycodon grandiflorum; Microsatellite markers; Genetic diversity; Conservation biology; SAPONINS; ROOTS;

D O I：

10.4238/2012.September.12.9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The balloon flower (Platycodon grandiflorum A. DC.) is a perennial flowering plant of the Campanulaceae family; it is the only member of the genus Platycodon. Information on the genetic diversity of balloon flower populations is of great importance for the conservation and germplasm utilization of this flowering plant. Twenty-two polymorphic microsatellite loci were developed and characterized with eight balloon flower accessions collected from South Korea and China. Eighty-one alleles were detected among the eight balloon flower accessions. The number of alleles per locus ranged from two to six, with a mean of four alleles per locus. The observed and expected heterozygosity values ranged from 0.000 to 0.875 (mean = 0.355) and 0.117 to 0.766 (mean = 0.489), respectively. The polymorphic information content values ranged from 0.110 to 0.733, with a mean of 0.449. These new microsatellite markers will be useful for population and conservation genetic studies of P. grandiflorum.

引用

页码：3263 / 3266

页数：4

共 10 条

[1] Five new triterpenoid saponins from the roots of Platycodon grandiflorum [J].

Fu, WW ;

Shimizu, N ;

Dou, DQ ;

Takeda, T ;

Fu, R ;

Pei, YH ;

Chen, YJ .

CHEMICAL & PHARMACEUTICAL BULLETIN, 2006, 54 (04) :557-560

[2] Diversity of root-associated bacteria associated with held-grown canola (Brassica napus L.) and wheat (Triticum aestivum L.) [J].

Germida, JJ ;

Siciliano, SD ;

de Freitas, JR ;

Seib, AM .

FEMS MICROBIOLOGY ECOLOGY, 1998, 26 (01) :43-50

[3]

Hong DY, 1983, SCI PRESS, V73, P76

[4]

ISHII H, 1978, CHEM PHARM BULL, V26, P674

[5]

Kim Kyonghui, 2004, MA thesis,

[6] Isolation of a new saponin and cytotoxic effect of saponins from the root of Platycodon grandiflorum on human tumor cell lines [J].

Kim, YS ;

Kim, JS ;

Choi, SU ;

Kim, JS ;

Lee, HS ;

Roh, SH ;

Jeong, YC ;

Kim, YK ;

Ryu, SY .

PLANTA MEDICA, 2005, 71 (06) :566-568

[7] Isolation and characterization of 21 microsatellite loci in Lycium chinense and cross-amplification in Lycium barbarum [J].

Kwon, Soon-Jae ;

Lee, Gi-An ;

Lee, Sok-Young ;

Park, Yong-Jin ;

Gwag, Jae-Gyun ;

Kim, Tae-San ;

Ma, Kyung-Ho .

CONSERVATION GENETICS, 2009, 10 (05) :1557-1560

[8] Antioxidant and anticancer activities of organic extracts from Platycodon grandiflorum A. De Candolle roots [J].

Lee, JY ;

Hwang, WI ;

Lim, ST .

JOURNAL OF ETHNOPHARMACOLOGY, 2004, 93 (2-3) :409-415

[9]

Liu K, 2005, POWERMARKER INTEGRAT

[10] An economic method for the fluorescent labeling of PCR fragments [J].

Schuelke, M .

NATURE BIOTECHNOLOGY, 2000, 18 (02) :233-234

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