RAE1 promotes BMAL1 shuttling and regulates degradation and activity of CLOCK: BMAL1 heterodimer

被引：21
作者：
Zheng, Xulei [1 ,2 ]
Zhao, Xu [1 ,2 ]
Zhang, Yingying [1 ,2 ]
Tan, Hao [1 ,2 ]
Qiu, Bojun [1 ,2 ]
Ma, Tengjiao [1 ,2 ]
Zeng, Jiarong [1 ,2 ]
Tao, Dachang [1 ,2 ]
Liu, Yunqiang [1 ,2 ]
Lu, Yilu [1 ,2 ]
Ma, Yongxin [1 ,2 ]
机构：
[1] Sichuan Univ, Dept Med Genet, State Key Lab Biotherapy, West China Hosp, Chengdu 610041, Peoples R China
[2] Collaborat Innovat Ctr, Chengdu 610041, Sichuan, Peoples R China
来源：
CELL DEATH & DISEASE | 2019年 / 10卷 / 2期
基金：
中国国家自然科学基金;
关键词：
CIRCADIAN CLOCK; COMPLEX; PROTEIN; LOCALIZATION; MECHANISMS; COMPONENTS; LIGHT; BUB3; LIMB;
D O I：
10.1038/s41419-019-1346-2
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Circadian rhythm is an autoregulatory rhythm, which is sustained by various mechanisms. The nucleocytoplasmic shuttling of BMAL1 is essential for CLOCK translocation between cytoplasm and nucleus and maintenance of the correct pace of the circadian clock. Here we showed that RAE1 and NUP98 can promote the degradation of BMAL1 and CLOCK. Knockdown of RAE1 and NUP98 suppressed BMAL1 shuttling, leading to cytoplasm accumulation of CLOCK. Furthermore, Chip assay showed that knockdown of RAE1 and NUP98 can enhance the interaction between CLOCK: BMAL1 and E-box region in the promoters of Per2 and Cry1 while reducing its transcription activation activity. Our present study firstly revealed that RAE1 and NUP98 are critical regulators for BMAL1 shuttling.
引用
收藏
页数：12
相关论文
共 31 条
  • [1] Circadian rhythm and light responsiveness of BMAL1 expression, a partner of mammalian clock gene Clock, in the suprachiasmatic nucleus of rats
    Abe, H
    Honma, S
    Namihira, M
    Tanahashi, Y
    Ikeda, M
    Honma, K
    [J]. NEUROSCIENCE LETTERS, 1998, 258 (02) : 93 - 96
  • [2] Rae1 is an essential mitotic checkpoint regulator that cooperates with Bub3 to prevent chromosome missegregation
    Babu, JR
    Jeganathan, KB
    Baker, DJ
    Wu, XS
    Kang-Decker, N
    van Deursen, JM
    [J]. JOURNAL OF CELL BIOLOGY, 2003, 160 (03) : 341 - 353
  • [3] Circadian Integration of Metabolism and Energetics
    Bass, Joseph
    Takahashi, Joseph S.
    [J]. SCIENCE, 2010, 330 (6009) : 1349 - 1354
  • [4] A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly
    Blower, MD
    Nachury, M
    Heald, R
    Weis, K
    [J]. CELL, 2005, 121 (02) : 223 - 234
  • [5] Proteomic analysis of the mammalian nuclear pore complex
    Cronshaw, JA
    Krutchinsky, AN
    Zhang, WZ
    Chait, BT
    Matunis, MJ
    [J]. JOURNAL OF CELL BIOLOGY, 2002, 158 (05) : 915 - 927
  • [6] Characterization of the Core Mammalian Clock Component, NPAS2, as a REV-ERBα/RORα Target Gene
    Crumbley, Christine
    Wang, Yongjun
    Kojetin, Douglas J.
    Burris, Thomas P.
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (46) : 35386 - 35392
  • [7] Role of the CLOCK protein in the mammalian circadian mechanism
    Gekakis, N
    Staknis, D
    Nguyen, HB
    Davis, FC
    Wilsbacher, LD
    King, DP
    Takahashi, JS
    Weitz, CJ
    [J]. SCIENCE, 1998, 280 (5369) : 1564 - 1569
  • [8] The Rae1-Nup98 complex prevents aneuploidy by inhibiting securin degradation
    Jeganathan, KB
    Malureanu, L
    van Deursen, JM
    [J]. NATURE, 2005, 438 (7070) : 1036 - 1039
  • [9] Detection of a circadian enhancer in the mDbp promoter using prokaryotic transposon vector-based strategy
    Kiyohara, Yota B.
    Nishii, Keigo
    Ukai-Tadenuma, Maki
    Ueda, Hiroki R.
    Uchiyama, Yasuo
    Yagita, Kazuhiro
    [J]. NUCLEIC ACIDS RESEARCH, 2008, 36 (04)
  • [10] Molecular components of the mammalian circadian clock
    Ko, Caroline H.
    Takahashi, Joseph S.
    [J]. HUMAN MOLECULAR GENETICS, 2006, 15 : R271 - R277
1234