Time-dependent biodistribution and excretion of silver nanoparticles in male Wistar rats

被引：196

作者：

Dziendzikowska, K. ^{[1
]}

Gromadzka-Ostrowska, J. ^{[1
]}

Lankoff, A. ^{[2
,3
]}

Oczkowski, M. ^{[1
]}

Krawczynska, A. ^{[1
]}

Chwastowska, J. ^{[3
]}

Sadowska-Bratek, M. ^{[3
]}

Chajduk, E. ^{[3
]}

Wojewodzka, M. ^{[3
]}

Dusinska, M. ^{[4
]}

Kruszewski, M. ^{[3
,5
]}

机构：

[1] Warsaw Univ Life Sci, Warsaw, Poland

[2] Jan Kochanowski Univ, Kielce, Poland

[3] Inst Nucl Chem & Technol, PL-03195 Warsaw, Poland

[4] Norwegian Inst Air Res, Kjeller, Norway

[5] Inst Rural Hlth, Lublin, Poland

来源：

JOURNAL OF APPLIED TOXICOLOGY | 2012年 / 32卷 / 11期

关键词：

nanoparticle accumulation; tissue distribution; cellular uptake; electron microscopy; mass spectrometry; BLOOD-BRAIN-BARRIER; TISSUE DISTRIBUTION; INHALATION TOXICITY; GOLD NANOPARTICLES; IN-VIVO; EXPOSURE;

D O I：

10.1002/jat.2758

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Silver nanoparticles (AgNPs) are the most commonly used nanoparticles owing to their antimicrobial properties. The motivation of the present study was (1) to analyze the effect of silver particle size on rat tissue distribution at different time points, (2) to determine the accumulation of AgNPs in potential rat target organs, (3) to analyze the intracellular distribution of AgNPs and (4) to examine the excretion of AgNPs by urine and feces. AgNPs were characterized by dynamic light scattering (DLS), zeta potential measurements, BET surface area measurements, transmission and scanning electron microscopy. AgNPs (20 and 200?nm) were administered intravenously (i.v.) to male Wistar rats at a dose of 5?mg?kg1 of body weight. Biological material was sampled 24?h, 7 and 28?days after injection. Using inductively coupled plasma-mass spectrometry (ICP-MS) and transmission electron microscopy (TEM) it was observed that AgNPs translocated from the blood to the main organs and the concentration of silver in tissues was significantly higher in rats treated with 20?nm AgNPs as compared with 200?nm AgNPs. The highest concentration of silver was found in the liver after 24?h. After 7?days, a high level of silver was observed in the lungs and spleen. The silver concentration in the kidneys and brain increased during the experiment and reached the highest concentration after 28?days. Moreover, the highest concentration of AgNPs was observed in the urine 1?day after the injection, maintained high for 14?days and then decreased. The fecal level of silver in rats was the highest within 2?days after AgNPs administration and then decreased. Copyright (c) 2012 John Wiley & Sons, Ltd.

引用

页码：920 / 928

页数：9

共 46 条

[1] The appearance of renal cells cytoplasmic degeneration and nuclear destruction might be an indication of GNPs toxicity [J].