Molecular Architecture of Plant Thylakoids under Physiological and Light Stress Conditions: A Study of Lipid-Light-Harvesting Complex II Model Membranes

被引：63

作者：

Janik, Ewa ^{[1
]}

Bednarska, Joanna ^{[1
]}

Zubik, Monika ^{[1
]}

Puzio, Michal ^{[1
]}

Luchowski, Rafal ^{[1
]}

Grudzinski, Wojciech ^{[1
]}

Mazur, Radoslaw ^{[2
]}

Garstka, Maciej ^{[2
]}

Maksymiec, Waldemar ^{[3
]}

Kulik, Andrzej ^{[4
]}

Dietler, Giovanni ^{[4
]}

Gruszecki, Wieslaw I. ^{[1
]}

机构：

[1] Marie Curie Sklodowska Univ, Inst Phys, Dept Biophys, PL-20031 Lublin, Poland

[2] Univ Warsaw, Fac Biol, Inst Biochem, Dept Metab Regulat, PL-02096 Warsaw, Poland

[3] Marie Curie Sklodowska Univ, Inst Biol & Biochem, Dept Plant Physiol, PL-20033 Lublin, Poland

[4] Ecole Polytech Fed Lausanne, Inst Phys Syst Biol, Lab Phys Mat Vivante, CH-1015 Lausanne, Switzerland

来源：

PLANT CELL | 2013年 / 25卷 / 06期

关键词：

OPTICALLY INDUCED REORGANIZATIONS; PHOTOSYSTEM-II; PROTEIN-PHOSPHORYLATION; CHLOROPLAST STRUCTURE; STATE TRANSITIONS; PHOTOSYNTHETIC MEMBRANES; TEMPERATURE-DEPENDENCE; ENERGY-DISSIPATION; NONBILAYER LIPIDS; CHLOROPHYLL;

D O I：

10.1105/tpc.113.113076

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, we analyzed multibilayer lipid-protein membranes composed of the photosynthetic light-harvesting complex II (LHCII; isolated from spinach [Spinacia oleracea]) and the plant lipids monogalcatosyldiacylglycerol and digalactosyldiacylglycerol. Two types of pigment-protein complexes were analyzed: those isolated from dark-adapted leaves (LHCII) and those from leaves preilluminated with high-intensity light (LHCII-HL). The LHCII-HL complexes were found to be partially phosphorylated and contained zeaxanthin. The results of the x-ray diffraction, infrared imaging microscopy, confocal laser scanning microscopy, and transmission electron microscopy revealed that lipid-LHCII membranes assemble into planar multibilayers, in contrast with the lipid-LHCII-HL membranes, which form less ordered structures. In both systems, the protein formed supramolecular structures. In the case of LHCII-HL, these structures spanned the multibilayer membranes and were perpendicular to the membrane plane, whereas in LHCII, the structures were lamellar and within the plane of the membranes. Lamellar aggregates of LHCII-HL have been shown, by fluorescence lifetime imaging microscopy, to be particularly active in excitation energy quenching. Both types of structures were stabilized by intermolecular hydrogen bonds. We conclude that the formation of trans-layer, rivet-like structures of LHCII is an important determinant underlying the spontaneous formation and stabilization of the thylakoid grana structures, since the lamellar aggregates are well suited to dissipate excess energy upon overexcitation.

引用

页码：2155 / 2170

页数：16