Screening of the human tumor necrosis factor-alpha (TNF-α) gene promoter polymorphisms by PCR-DGGE analysis

被引：12
|
作者：
Patiño-García, A
Sotillo-Piñeiro, E
Modesto, C
Sierrasesúmaga, L
机构：
[1] Univ Navarra, Univ Clin, Dept Paediat, E-31080 Pamplona, Spain
[2] Hosp Gen Valle Hebron, Dept Internal Med, Barcelona, Spain
来源：
MUTATION RESEARCH-GENOMICS | 1999年 / 406卷 / 2-4期
关键词：
PCR; DGGE; polymorphism; TNF-alpha;
D O I：
10.1016/S1383-5726(99)00004-7
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We have designed a new PCR-DGGE technique that enables detection of base changes in the TNF-alpha gene promoter. Screening of 130 samples from Spanish children has shown that this technique accurately detects the altered band patterns induced by the presence of the polymorphisms at positions -376, -308, -238 and -163 of the promoter sequence. Although further analysis are needed to fully characterise the alterations detected, we believe that this PCR-DGGE technique is a rapid and sensitive first approach to the genetic characterisation of the TNF-alpha promoter. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：121 / 125
页数：5
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