M51R and Delta-M51 matrix protein of the vesicular stomatitis virus induce apoptosis in colorectal cancer cells

被引:17
作者
Gray, Zahra [1 ]
Tabarraei, Alijan [1 ]
Moradi, Abdolvahab [1 ]
Kalani, Mohamad R. [2 ,3 ]
机构
[1] Golestan Univ Med Sci, Coll Med, Dept Microbiol, 1 Shastcola Ave,5 Km Sari Rd, Gorgan, Golestan, Iran
[2] Golestan Univ Med Sci, Cell & Mol Res Ctr, 1 Shastcola Ave,5 Km Sari Rd, Gorgan, Golestan, Iran
[3] Univ Illinois, RAL, Mol & Cell Biol, 600 S Goodwin Ave 325, Urbana, IL 61801 USA
关键词
VSV; Oncolytic virus; Apoptosis; Colon cancer; GENE-EXPRESSION; WILD-TYPE; MUTANT; SENSITIVITY; VIROTHERAPY; SOCIETY; ADULTS; COLON;
D O I
10.1007/s11033-019-04799-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Colorectal cancer (CRC) is the third most common cancer in both men and women. Oncolytic viral-based therapy methods seem to be promising for CRC treatment. Vesicular stomatitis virus (VSV) is considered as a potent candidate in viral therapy for several tumors. VSV particles with mutated matrix (M) protein are capable of initiating cell death cascades while not being harmful to the immune system. In the current study, the effects of the VSV M-protein was investigated on the apoptosis of the colorectal cancer SW480 cell. Wild-type, M51R, and M51 mutants VSV M-protein genes were cloned into the PCDNA3.1 vector and transfected into the SW480 cells. The results of the MTT assay, Western blotting, and Caspase 3, 8, and 9 measurement, illustrated that both wild and M51R mutant M-proteins can destroy the SW480 colorectal cancer cells. DAPI/TUNEL double-staining reconfirmed the apoptotic effects of the M-protein expression. The M51 mutant M-protein is effective likewise M51R, somehow it can be considered as a safer substitution.
引用
收藏
页码:3371 / 3379
页数:9
相关论文
共 48 条
  • [1] Sensitivity of prostate tumors to wild type and M protein mutant vesicular stomatitis viruses
    Ahmed, M
    Cramer, SD
    Lyles, DS
    [J]. VIROLOGY, 2004, 330 (01) : 34 - 49
  • [2] Defective translational control facilitates vesicular stomatitis virus oncolysis
    Balachandran, S
    Barber, GN
    [J]. CANCER CELL, 2004, 5 (01) : 51 - 65
  • [3] Characterization of the Interaction between the Matrix Protein of Vesicular Stomatitis Virus and the Immunoproteasome Subunit LMP2
    Beilstein, Frauke
    Obiang, Linda
    Raux, Helene
    Gaudin, Yves
    [J]. JOURNAL OF VIROLOGY, 2015, 89 (21) : 11019 - 11029
  • [4] Oncotargeting by Vesicular Stomatitis Virus (VSV): Advances in Cancer Therapy
    Bishnoi, Suman
    Tiwari, Ritudhwaj
    Gupta, Sharad
    Byrareddy, Siddappa N.
    Nayak, Debasis
    [J]. VIRUSES-BASEL, 2018, 10 (02):
  • [5] THE ROLE OF VESICULAR STOMATITIS-VIRUS MATRIX PROTEIN IN INHIBITION OF HOST-DIRECTED GENE-EXPRESSION IS GENETICALLY SEPARABLE FROM ITS FUNCTION IN VIRUS ASSEMBLY
    BLACK, BL
    RHODES, RB
    MCKENZIE, M
    LYLES, DS
    [J]. JOURNAL OF VIROLOGY, 1993, 67 (08) : 4814 - 4821
  • [6] Oncolytic Vesicular Stomatitis Virus Induces Apoptosis in U87 Glioblastoma Cells by a Type II Death Receptor Mechanism and Induces Cell Death and Tumor Clearance In Vivo
    Cary, Zachary D.
    Willingham, Mark C.
    Lyles, Douglas S.
    [J]. JOURNAL OF VIROLOGY, 2011, 85 (12) : 5708 - 5717
  • [7] Proteomic approach to studying the cytotoxicity of YCA on U937 leukemia cells and antileukernia activity in orthotopic model of leukemia mice
    Chung, Jing-Gung
    Yang, Jai-Sing
    Huang, Li-Jiau
    Lee, Fang-Yu
    Teng, Che-Ming
    Tsai, Sheng-Chung
    Lin, Kuei-Li
    Wang, Shu-Fang
    Kuo, Sheng-Chu
    [J]. PROTEOMICS, 2007, 7 (18) : 3305 - 3317
  • [8] Impaired interferon signaling is a common immune defect in human cancer
    Critchley-Thorne, Rebecca J.
    Simons, Diana L.
    Yan, Ning
    Miyahira, Andrea K.
    Dirbas, Frederick M.
    Johnson, Denise L.
    Swetter, Susan M.
    Carlson, Robert W.
    Fisher, George A.
    Koong, Albert
    Holmes, Susan
    Lee, Peter P.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (22) : 9010 - 9015
  • [9] IFN-β-Induced Alteration of VSV Protein Phosphorylation in Neuronal Cells
    D'agostino, Paul M.
    Amenta, Jessica J.
    Reiss, Carol Shoshkes
    [J]. VIRAL IMMUNOLOGY, 2009, 22 (06) : 353 - 369
  • [10] Increased colorectal cancer incidence in Iran: a systematic review and meta-analysis
    Dolatkhah, Roya
    Somi, Mohammad Hossein
    Kermani, Iraj Asvadi
    Ghojazadeh, Morteza
    Jafarabadi, Mohamad Asghari
    Farassati, Faris
    Dastgiri, Saeed
    [J]. BMC PUBLIC HEALTH, 2015, 15