Flow cytometry: A new method to investigate the properties of water-in-oil-in-water emulsions

被引：34

作者：

Hai, M

Bernath, K

Tawfik, D

Magdassi, S ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Casali Inst Appl Chem, IL-91904 Jerusalem, Israel

[2] Weizmann Inst Sci, Dept Biol Chem, IL-76100 Rehovot, Israel

来源：

LANGMUIR | 2004年 / 20卷 / 06期

关键词：

D O I：

10.1021/la035402+

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We present a new and facile method to evaluate w/o/w emulsions containing fluorescent markers by flow cytometry. Flow cytometry allows simultaneous measurement of w/o/w emulsion droplets "marked" with a fluorescent marker or "blank" without the need for complicated sample preparation. The yield of preparation of the w/o/w emulsion and the release rate of the fluorescent marker FITC-BSA were investigated by this new method. The release fraction (after 24 h) of FITC-BSA from the w/o/w emulsion decreased with increasing concentration of FITC-BSA inside the internal phase, just like the release fraction of NaCl as marker from the w/o/w emulsion. Flow cytometry results show that the yield and release behavior in w/o/w emulsions are in agreement with results reported by more complicated methods.

引用

页码：2081 / 2085

页数：5

共 17 条

[1] Optimizing and quantifying fusion of liposomes to mammalian sperm using resonance energy transfer and flow cytometric methods [J].