Microbiota-Derived Extracellular Vesicles Detected in Human Blood from Healthy Donors

被引：26
作者：
Schaack, Beatrice [1 ,2 ]
Hindre, Thomas [1 ]
Quansah, Nyamekye [1 ]
Hannani, Dalil [1 ]
Mercier, Corinne [1 ]
Laurin, David [3 ,4 ,5 ]
机构：
[1] Univ Grenoble Alpes, TIMC, Grenoble INP, VetAgro Sup,UMR 5525,CNRS, F-38000 Grenoble, France
[2] Univ Grenoble Alpes, IBS, CNRS, CEA, F-38044 Grenoble, France
[3] Dept Sci Auvergne Rhone Alpes, Etab Francais Sang, F-38000 Grenoble, France
[4] Univ Grenoble Alpes, Inst Adv Biosci, INSERM, U1209, F-38000 Grenoble, France
[5] Univ Grenoble Alpes, Inst Adv Biosci, CNRS, UMR 5309, F-38000 Grenoble, France
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 22期
关键词：
extracellular vesicles (EVs); outer membrane vesicles (OMVs); membrane fusion; red blood cell concentrates; lipopolysaccharide (LPS); OmpA; gut microbiota; OUTER-MEMBRANE VESICLES; LIPOPOLYSACCHARIDE; AGGREGATION;
D O I：
10.3390/ijms232213787
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The microbiota constitutes an important part of the holobiont in which extracellular vesicles (EVs) are key players in health, especially regarding inter- and intra-kingdom communications. Analysis of EVs from the red blood cell concentrates of healthy donors revealed variable amounts of OmpA and LPS in 12 of the 14 analyzed samples, providing indirect experimental evidence of the presence of microbiota EVs in human circulating blood in the absence of barrier disruption. To investigate the role of these microbiota EVs, we tracked the fusion of fluorescent Escherichia coli EVs with blood mononuclear cells and showed that, in the circulating blood, these EVs interacted almost exclusively with monocytes. This study demonstrates that bacterial EVs constitute critical elements of the host-microbiota cellular communication. The analysis of bacterial EVs should thus be systematically included in any characterization of human EVs.
引用
收藏
页数：12
相关论文
共 30 条
  • [1] Outer Membrane Vesicles and Soluble Factors Released by Probiotic Escherichia coil Nissle 1917 and Commensal ECOR63 Enhance Barrier Function by Regulating Expression of Tight Junction Proteins in Intestinal Epithelial Cells
    Alvarez, Carina-Shianya
    Badia, Josefa
    Bosch, Manel
    Gimenez, Rosa
    Baldoma, Laura
    [J]. FRONTIERS IN MICROBIOLOGY, 2016, 7
  • [2] Caspase-4 disaggregates lipopolysaccharide micelles via LPS-CARD interaction
    An, Jinsu
    Kim, Seong Ho
    Hwang, Dohyeon
    Lee, Kyung Eun
    Kim, Min Jung
    Yang, Eun Gyeong
    Kim, So Yeon
    Chung, Hak Suk
    [J]. SCIENTIFIC REPORTS, 2019, 9 (1)
  • [3] Critical aggregation concentrations of gram-negative bacterial lipopolysaccharides (LPS)
    Aurell, CA
    Wistrom, AO
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 253 (01) : 119 - 123
  • [4] Outer Membrane Vesicles of Gram-Negative Bacteria: An Outlook on Biogenesis
    Avila-Calderon, Eric Daniel
    del Socorro Ruiz-Palma, Maria
    Aguilera-Arreola, Ma. Guadalupe
    Velazquez-Guadarrama, Norma
    Ruiz, Enrico A.
    Gomez-Lunar, Zulema
    Witonsky, Sharon
    Contreras-Rodriguez, Araceli
    [J]. FRONTIERS IN MICROBIOLOGY, 2021, 12
  • [5] Microbiota-Derived Extracellular Vesicles as New Systemic Regulators
    Badi, Sara Ahmadi
    Moshiri, Arfa
    Fateh, Abolfazl
    Jamnani, Fatemeh Rahimi
    Sarshar, Meysam
    Vaziri, Farzam
    Siadat, Seyed Davar
    [J]. FRONTIERS IN MICROBIOLOGY, 2017, 8
  • [6] Protein selection and export via outer membrane vesicles
    Bonnington, K. E.
    Kuehn, M. J.
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2014, 1843 (08): : 1612 - 1619
  • [7] Emerging role of bacterial extracellular vesicles in cancer
    Chronopoulos, Antonios
    Kalluri, Raghu
    [J]. ONCOGENE, 2020, 39 (46) : 6951 - 6960
  • [8] Naturally Produced Outer Membrane Vesicles from Pseudomonas aeruginosa Elicit a Potent Innate Immune Response via Combined Sensing of Both Lipopolysaccharide and Protein Components
    Ellis, Terri N.
    Leiman, Sara A.
    Kuehn, Meta J.
    [J]. INFECTION AND IMMUNITY, 2010, 78 (09) : 3822 - 3831
  • [9] Role of Vγ9vδ2 T lymphocytes in infectious diseases
    Gay, Laetitia
    Mezouar, Soraya
    Cano, Carla
    Frohna, Paul
    Madakamutil, Loui
    Mege, Jean-Louis
    Olive, Daniel
    [J]. FRONTIERS IN IMMUNOLOGY, 2022, 13
  • [10] Potent Bidirectional Cross-Talk Between Plasmacytoid Dendritic Cells and γδT Cells Through BTN3A, Type I/II IFNs and Immune Checkpoints
    Girard, Pauline
    Ponsard, Benedicte
    Charles, Julie
    Chaperot, Laurence
    Aspord, Caroline
    [J]. FRONTIERS IN IMMUNOLOGY, 2020, 11
123