Microbiota-Derived Extracellular Vesicles Detected in Human Blood from Healthy Donors

被引：31

作者：

Schaack, Beatrice ^{[1
,2
]}

Hindre, Thomas ^{[1
]}

Quansah, Nyamekye ^{[1
]}

Hannani, Dalil ^{[1
]}

Mercier, Corinne ^{[1
]}

Laurin, David ^{[3
,4
,5
]}

机构：

[1] Univ Grenoble Alpes, TIMC, Grenoble INP, VetAgro Sup,UMR 5525,CNRS, F-38000 Grenoble, France

[2] Univ Grenoble Alpes, IBS, CNRS, CEA, F-38044 Grenoble, France

[3] Dept Sci Auvergne Rhone Alpes, Etab Francais Sang, F-38000 Grenoble, France

[4] Univ Grenoble Alpes, Inst Adv Biosci, INSERM, U1209, F-38000 Grenoble, France

[5] Univ Grenoble Alpes, Inst Adv Biosci, CNRS, UMR 5309, F-38000 Grenoble, France

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 22期

关键词：

extracellular vesicles (EVs); outer membrane vesicles (OMVs); membrane fusion; red blood cell concentrates; lipopolysaccharide (LPS); OmpA; gut microbiota; OUTER-MEMBRANE VESICLES; LIPOPOLYSACCHARIDE; AGGREGATION;

D O I：

10.3390/ijms232213787

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The microbiota constitutes an important part of the holobiont in which extracellular vesicles (EVs) are key players in health, especially regarding inter- and intra-kingdom communications. Analysis of EVs from the red blood cell concentrates of healthy donors revealed variable amounts of OmpA and LPS in 12 of the 14 analyzed samples, providing indirect experimental evidence of the presence of microbiota EVs in human circulating blood in the absence of barrier disruption. To investigate the role of these microbiota EVs, we tracked the fusion of fluorescent Escherichia coli EVs with blood mononuclear cells and showed that, in the circulating blood, these EVs interacted almost exclusively with monocytes. This study demonstrates that bacterial EVs constitute critical elements of the host-microbiota cellular communication. The analysis of bacterial EVs should thus be systematically included in any characterization of human EVs.

引用

页数：12

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