Short Report: Polymerase Chain Reaction for Chronic Trypanosoma cruzi Infection Yields Higher Sensitivity in Blood Clot Than Buffy Coat or Whole Blood Specimens

被引：33

作者：

Fitzwater, Sean ^{[2
]}

Calderon, Maritza ^{[3
]}

LaFuente, Carlos ^{[4
]}

Galdos-Cardenas, Gerson ^{[2
]}

Ferrufino, Lisbeth ^{[4
]}

Verastegui, Manuela ^{[3
]}

Gilman, Robert H. ^{[2
]}

Bern, Caryn ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Div Parasit Dis, Natl Ctr Zoonot Vector Borne & Enter Dis, Atlanta, GA 30341 USA

[2] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Int Hlth, Baltimore, MD 21205 USA

[3] Univ Peruana Cayetano Heredia, Fac Ciencias & Filosofia, Lab Enfermedades Infecciosas, Lima, Peru

[4] Hosp Univ Japones, Santa Cruz, Bolivia

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2008年 / 79卷 / 05期

关键词：

D O I：

10.4269/ajtmh.2008.79.768

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Trypanosoma cruzi polymerase chain reaction (PCR) is widely used, but sensitivity varies widely. We compared PCR using 121/122 primers targeting kinetoplast minicircle DNA in whole blood, buffy coat, and clot from Bolivian women. Sensitivity was significantly higher in clot (60.1 %) than buffy coat (46.5%) or whole blood (40%). The use of clot could simplify specimen collection while improving sensitivity.

引用

页码：768 / 770

页数：3

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