Knockdown of long non-coding RNA CCAT2 suppressed proliferation and migration of glioma cells

被引：55
|
作者：
Guo, Hua [1 ]
Hu, Guowen [1 ]
Yang, Qing [2 ]
Zhang, Pei [1 ]
Kuang, Wei [1 ]
Zhu, Xingen [1 ]
Wu, Lei [1 ]
机构：
[1] Nanchang Univ, Affiliated Hosp 2, Dept Neurosurg, Nanchang 330006, Peoples R China
[2] Nanchang Univ, Affiliated Hosp 2, Dept Resp Med, Nanchang 330006, Peoples R China
来源：
ONCOTARGET | 2016年 / 7卷 / 49期
关键词：
qRT-PCR; quantitative real-time PCR; lncRNA; long non-coding RNA (lncRNAs); CCK-8; cell counting kit-8; GASTRIC-CANCER; BREAST-CANCER; CARCINOMA; EXPRESSION; GROWTH; OVEREXPRESSION; ACTIVATION; INVASION;
D O I：
10.18632/oncotarget.13242
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Long non-coding RNA colon cancer-associated transcript 2 (CCAT2) is commonly investigated in a number of cancers. However, little is known of its expression and biological function in glioma biology. In the current study, we used quantitative real-time PCR (qRT-PCR) to determine the expression of CCAT2 in glioma tissues. We found that expression of CCAT2 was up-regulated in glioma tissues and significantly correlated with the advanced tumor stage (III/IV). Functional assays in vitro and in vivo demonstrated that knockdown of CCAT2 could inhibit proliferation, cell cycle progression and migration of glioma cells. Further analysis indicated the effect of CCAT2 knockdown on glioma cell phenotype through inhibiting Wnt/beta-catenin signal pathway activity. Thus, our study provides evidence that CCAT2 may function as a potential biomarker for glioma.
引用
收藏
页码：81806 / 81814
页数：9
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