Reduced graphene oxide-Sb2O5 hybrid nanomaterial for the design of a laccase-based amperometric biosensor for estriol

被引：51
作者：
Cincotto, Fernando H. [1 ,2 ]
Canevari, Thiago C. [3 ]
Machado, Sergio A. S. [2 ]
Sanchez, Alfredo [1 ]
Ruiz Barrio, Maria Asuncion [1 ]
Villalonga, Reynaldo [1 ]
Pingarron, Jose M. [1 ]
机构：
[1] Univ Complutense Madrid, Fac Chem, Dept Analyt Chem, E-28040 Madrid, Spain
[2] State Univ Sao Paulo, Inst Chem, BR-13560970 Sao Carlos, SP, Brazil
[3] Mackenzie Presbiterian Univ, Sch Engn, BR-01302907 Sao Paulo, SP, Brazil
来源：
ELECTROCHIMICA ACTA | 2015年 / 174卷
基金：
巴西圣保罗研究基金会;
关键词：
Biosensor; estriol; graphene oxide; Sb2O5; hybrid material; GLASSY-CARBON ELECTRODE; ELECTROCHEMICAL BIOSENSOR; LIQUID-CHROMATOGRAPHY; MASS-SPECTROMETRY; BISPHENOL-A; OXIDE; NANOPARTICLES; ESTROGENS; FILM; IMMUNOASSAY;
D O I：
10.1016/j.electacta.2015.06.013
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
A novel reduced graphene oxide/Sb2O5 hybrid nanomaterial was prepared by a one-pot reaction process. The nanomaterial was characterized by different spectroscopic, microscopic and electrochemical techniques, demonstrating that the graphene sheets were doped with a Sb2O5 thin film. A glassy carbon electrode coated with the hybrid material was further employed as support for the covalent immobilization of laccase to develop an electrochemical biosensor for estriol. The enzyme biosensor showed high sensitivity (275 mA/M) and fast analytical response (4 s) for the hormone, with a limit of detection of 11 nM in the range of 25 nM to 1.03 p,M. The biosensor showed high selectivity for the analytical detection of estriol. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：332 / 339
页数：8
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