Predicting the Presence of Non-O157 Shiga Toxin-Producing Escherichia coli in Ground Beef by Using Molecular Tests for Shiga Toxins, Intimin, and O Serogroups

被引:30
作者
Bosilevac, Joseph M. [1 ]
Koohmaraie, Mohammad [2 ,3 ]
机构
[1] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA
[2] IEH Labs & Consulting Grp, Lake Forest Pk, WA USA
[3] King Saud Univ, Coll Food & Agr, Riyadh, Saudi Arabia
关键词
PCR AMPLIFICATION; UNITED-STATES; GENE-CLUSTER; INFECTIONS; ANTIGEN; WZX; SEQUENCE; DIARRHEA; ASSAYS;
D O I
10.1128/AEM.01508-12
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
When 3,972 ground beef enrichments with 6 confirmed to contain a non-O157 Shiga toxin-producing intimin-positive Escherichia coli isolate were tested for Shiga toxin, intimin, and O group (O26, O45, O103, O111, O121, and O145) genes, 183 potential positives and only 2 of the 6 confirmed positives were identified.
引用
收藏
页码:7152 / 7155
页数:4
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