Predicting the Presence of Non-O157 Shiga Toxin-Producing Escherichia coli in Ground Beef by Using Molecular Tests for Shiga Toxins, Intimin, and O Serogroups

被引：30
作者：
Bosilevac, Joseph M. [1 ]
Koohmaraie, Mohammad [2 ,3 ]
机构：
[1] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA
[2] IEH Labs & Consulting Grp, Lake Forest Pk, WA USA
[3] King Saud Univ, Coll Food & Agr, Riyadh, Saudi Arabia
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2012年 / 78卷 / 19期
关键词：
PCR AMPLIFICATION; UNITED-STATES; GENE-CLUSTER; INFECTIONS; ANTIGEN; WZX; SEQUENCE; DIARRHEA; ASSAYS;
D O I：
10.1128/AEM.01508-12
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
When 3,972 ground beef enrichments with 6 confirmed to contain a non-O157 Shiga toxin-producing intimin-positive Escherichia coli isolate were tested for Shiga toxin, intimin, and O group (O26, O45, O103, O111, O121, and O145) genes, 183 potential positives and only 2 of the 6 confirmed positives were identified.
引用
收藏
页码：7152 / 7155
页数：4
相关论文
共 20 条
[1]   Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina [J].
Alejandra Rivero, Mariana ;
Antonio Passucci, Juan ;
Mario Rodriguez, Edgardo ;
Ernesto Parma, Alberto .
JOURNAL OF MEDICAL MICROBIOLOGY, 2010, 59 (03) :345-352
[2]  
[Anonymous], 2011, Fed. Regist, V76, P58157
[3]   Association of Escherichia coli O157:H7 tir polymorphisms with human infection [J].
Bono, James L. ;
Keen, James E. ;
Clawson, Michael L. ;
Durso, Lisa M. ;
Heaton, Michael P. ;
Laegreid, William W. .
BMC INFECTIOUS DISEASES, 2007, 7 (1)
[4]  
Bosilevac JM, 2009, 7 INT S SHIG TOX VER
[5]   Prevalence and Characterization of Non-O157 Shiga Toxin-Producing Escherichia coli Isolates from Commercial Ground Beef in the United States [J].
Bosilevac, Joseph M. ;
Koohmaraie, Mohammad .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2011, 77 (06) :2103-2112
[6]   Non-O157 shiga toxin-producing Escherichia coli infections in the United States, 1983-2002 [J].
Brooks, JT ;
Sowers, EG ;
Wells, JG ;
Greene, KD ;
Griffin, PM ;
Hoekstra, RM ;
Strockbine, NA .
JOURNAL OF INFECTIOUS DISEASES, 2005, 192 (08) :1422-1429
[7]   Molecular analysis as an aid to assess the public health risk of non-O157 Shiga toxin-producing Escherichia coli strains [J].
Coombes, Brian K. ;
Wickham, Mark E. ;
Mascarenhas, Marjola ;
Gruenheid, Samantha ;
Finlay, B. Brett ;
Karmali, Mohamed A. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (07) :2153-2160
[8]   Detection of Escherichia coli serogroups O26 and O113 by PCR amplification of the wzx and wzy genes [J].
DebRoy, C ;
Roberts, E ;
Kundrat, J ;
Davis, MA ;
Briggs, CE ;
Fratamico, PM .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (03) :1830-1832
[9]   Development of PCR assays targeting genes in O-antigen gene clusters for detection and identification of Escherichia coli O45 and O55 serogroups [J].
DebRoy, C ;
Fratamico, PM ;
Roberts, E ;
Davis, MA ;
Liu, YH .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (08) :4919-4924
[10]   Molecular serotyping of Escherichia coli O111:H8 [J].
Durso, Lisa M. ;
Bono, James L. ;
Keen, James E. .
JOURNAL OF MICROBIOLOGICAL METHODS, 2007, 69 (02) :381-383
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