High resolution melting analysis for quantitative detection of bovine milk in pure water buffalo mozzarella and other buffalo dairy products

被引：26

作者：

Sakaridis, Ioannis ^{[1
]}

Ganopoulos, Ioannis ^{[1
,2
]}

Argiriou, Anagnostis ^{[1
]}

Tsaftaris, Athanasios ^{[1
,2
]}

机构：

[1] CERTH, Inst Appl Biosci, Thessaloniki 57001, Greece

[2] Aristotle Univ Thessaloniki, Sch Agr, Dept Genet & Plant Breeding, Thessaloniki 54124, Greece

来源：

INTERNATIONAL DAIRY JOURNAL | 2013年 / 28卷 / 01期

关键词：

POLYMERASE-CHAIN-REACTION; SPECIES-SPECIFIC PCR; HRM ANALYSIS; SHEEPS MILK; COWS MILK; DNA; CHEESE; IDENTIFICATION; QUANTIFICATION; MICROSATELLITE;

D O I：

10.1016/j.idairyj.2012.08.006

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Identification of buffalo dairy products has become an important issue to ascertain product quality, consumer rights and absence of food-borne allergic reactions. A polymerase chain reaction (PCR) followed by a high resolution melting (HRM) analysis was developed and applied for species specific detection of bovine milk in nine different commercial buffalo dairy products. A specific buffalo 12S rRNA and a bovine D-loop primer pair, targeting the mitochondrial genome, were employed in a duplex PCR assay. The analysis developed was found capable of identifying the presence of bovine milk down to 1% in commercial buffalo milk products and also of quantifying the ratio of bovine into buffalo milk. HRM was proven to be a fast and accurate technique for a routine authentication testing of mozzarella and other buffalo milk products. (c) 2012 Elsevier Ltd. All rights reserved.

引用

页码：32 / 35

页数：4

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