The specific infectivity of hepatitis C virus changes through its life cycle

被引:24
作者
Keum, Sun Ju [1 ]
Park, Sung Mi [1 ]
Park, Ji Hoon [1 ]
Jung, Jong Ha [1 ]
Shin, Eun Ji [1 ]
Jang, Sung Key [1 ,2 ,3 ]
机构
[1] Pohang Univ Sci & Technol, POSTECH Biotech Ctr, Dept Life Sci, Pohang, South Korea
[2] Pohang Univ Sci & Technol, Div Integrat Biosci & Biotechnol, Pohang, South Korea
[3] Pohang Univ Sci & Technol, Biotechnol Res Ctr, Pohang, South Korea
基金
新加坡国家研究基金会;
关键词
Hepatitis C virus; One-step growth; The kinetics of HCV replication; The kinetics of HCV production; Chracterization of HCV particles; Specific infectivity of HCV; DENSITY-GRADIENT CENTRIFUGATION; APOLIPOPROTEIN-E; RNA REPLICATION; RT-PCR; BUOYANT DENSITY; CULTURE; CELLS; ASSAY; IMMUNOCOMPETENT; IMMUNODEFICIENT;
D O I
10.1016/j.virol.2012.08.046
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Hepatitis C virus (HCV) causes liver diseases, such as hepatitis, liver cirrhosis, steatosis, and hepatocellular carcinoma. To understand the life cycle and pathogenesis of HCV, the one-step growth of HCV in a cell culture system was analyzed using a highly infectious variant of the JFH1 clone. The observed profiles of HCV RNA replication indicated that the synthesis of negative-strand RNAs occurred at 6 h (h) after infection, followed by the active synthesis of positive-strand RNAs. Our measurements of infectious virus production showed that the latent period of HCV was about 12 h. The specific infectivity of HCV particles (focus-forming unit per viral RNA molecule) secreted to the extracellular milieu early in infection was about 30-fold higher than that secreted later during infection. The buoyant densities of the infectious virion particles differed with the duration of infection, indicating changes in the compositions of the virion particles. (C) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码:462 / 470
页数:9
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