Ca2+ homeostasis and Ca2+ signalling in sulphur mustard-exposed normal human epidermal keratinocytes

被引：18

作者：

Mol, MAE ^{[1
]}

Smith, WJ ^{[1
]}

机构：

[1] USA,MED RES INST CHEM DEF,ABERDEEN PROVING GROUND,MD 21010

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 1996年 / 100卷 / 01期

关键词：

human epidermal keratinocytes; sulphur mustard; cytoplasmic free calcium levels; Fura-2; signal transduction; histamine; ATP;

D O I：

10.1016/S0009-2797(96)03692-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To investigate the role of increased cytoplasmic free calcium levels ([Ca](i)) in sulphur mustard-induced cellular toxicity, the [Ca](i) of human epidermal keratinocytes exposed to sulphur mustard concentrations between 50 and 800 mu M were measured. No early increase in [Ca](i) was observed during the first 20 min after application of the agent. Furthermore, at 3, 6 and 24 h after exposure to sulphur mustard, the [Ca](i) of sulphur mustard-exposed cells differed only slightly from that of control cells. However, sulphur-mustard exposed cells responded with a weaker rise of [Ca](i) upon stimulation with histamine and ATP than control cells. These results suggest that sulphur mustard can induce small increases in [Ca](i), which reflect abnormal cellular physiology rather than acute toxicity. In addition, it has been shown that sulphur mustard disturbs cellular Ca2+ signalling in human epidermal keratinocytes.

引用

页码：85 / 93

页数：9

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