Application of aqueous hydrazine solution for β-elimination of O-glycans from gastric mucin

被引：2

作者：

Kisiel, D ^{[1
]}

Gindzienski, A ^{[1
]}

Gadek, A ^{[1
]}

机构：

[1] Med Acad Bialystok, Dept Gen & Organ Chem, Inst Chem, PL-15230 Bialystok 8, Poland

来源：

ACTA BIOCHIMICA POLONICA | 1999年 / 46卷 / 03期

关键词：

mucin; beta-elimination; aqueous hydrazine;

D O I：

10.18388/abp.1999_4147

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

O-Glycans from pig gastric mucin were released by beta-elimination in 0.2 M triethylamine and 50% aqueous hydrazine solution. The released glycan hydrazides were isolated using Centricon 10 separators, brought to their reducing form and reductive by labelled with p-aminobenzoic acid ethyl ester (ABEE). Labelled products were fractionated into neutral and acid fractions on a Bio-Gel P4 column, calibrated with a mixture of dextran oligosaccharides, labelled according to the same procedure.

引用

页码：753 / 757

页数：5

共 9 条

[1] QUANTITATION OF OLIGOSACCHARIDES RELEASED BY THE BETA-ELIMINATION REACTION [J].

AMINOFF, D ;

GATHMANN, WD ;

MCLEAN, CM ;

YADOMAE, T .

ANALYTICAL BIOCHEMISTRY, 1980, 101 (01) :44-53

[2]

CARLSON DM, 1968, J BIOL CHEM, V243, P616

[3] THE ELIMINATION OF O-LINKED GLYCANS FROM GLYCOPROTEINS UNDER NONREDUCING CONDITIONS [J].

COOPER, CA ;

PACKER, NH ;

REDMOND, JW .

GLYCOCONJUGATE JOURNAL, 1994, 11 (02) :163-167

[4] COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].

DUBOIS, M ;

GILLES, KA ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

ANALYTICAL CHEMISTRY, 1956, 28 (03) :350-356

[5]

GINDZIENSKI A, 1987, BIOMED BIOCHIM ACTA, V46, P165

[6] ANALYSIS OF MONOSACCHARIDE COMPOSITION OF MUCIN OLIGOSACCHARIDE-ALDITOLS BY HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY [J].

KARLSSON, NG ;

HANSSON, GC .

ANALYTICAL BIOCHEMISTRY, 1995, 224 (02) :538-541

[7] Analysis of O-linked oligosaccharide alditols by high-pH anion-exchange chromatography with pulsed amperometric detection [J].

Kotani, N ;

Takasaki, S .

ANALYTICAL BIOCHEMISTRY, 1997, 252 (01) :40-47

[8] CHROMATOGRAPHIC-SEPARATION OF ASPARAGINE-LINKED OLIGOSACCHARIDES LABELED WITH AN ULTRAVIOLET-ABSORBING COMPOUND, PARA-AMINOBENZOIC ACID ETHYL-ESTER [J].

MATSUURA, F ;

IMAOKA, A .

GLYCOCONJUGATE JOURNAL, 1988, 5 (01) :13-26

[9] USE OF HYDRAZINE TO RELEASE IN INTACT AND UNREDUCED FORM BOTH N-LINKED AND O-LINKED OLIGOSACCHARIDES FROM GLYCOPROTEINS [J].

PATEL, T ;

BRUCE, J ;

MERRY, A ;

BIGGE, C ;

WORMALD, M ;

JAQUES, A ;

PAREKH, R .

BIOCHEMISTRY, 1993, 32 (02) :679-693

← 1 →