Comperative study of catalase immobilization on chitosan, magnetic chitosan and chitosan-clay composite beads

被引：10
作者：
Basak, Esra [1 ]
Aydemir, Tulin [1 ]
Dincer, Ayse [1 ]
Becerik, Seda Cinar [1 ]
机构：
[1] Celal Bayar Univ, Fac Sci & Arts, Dept Chem, TR-45140 Muradiye, Manisa, Turkey
来源：
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | 2013年 / 41卷 / 06期
关键词：
catalase; composite beads; comparative study; immobilization; reusability; ADSORPTION; KINETICS;
D O I：
10.3109/10731199.2013.796312
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Catalase was immobilized on chitosan and modified chitosan. Studies were carried out on free-immobilized catalase concerning the determination of optimum temperature, pH, thermal, storage stability, reusability, and kinetic parameters. Optimum temperature and pH for free catalase and catalase immobilized were found as 35 degrees C and 7.0, respectively. After 100 times of repeated tests, the immobilized catalases on chitosan-clay and magnetic chitosan maintain over 50% and 60% of the original activity, respectively. The ease of catalase immobilization on low-cost matrices and good stability upon immobilization in the present study make it a suitable product for further use in the food industry.
引用
收藏
页码：408 / 413
页数：6
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