A highly sensitive europium nanoparticle-based lateral flow immunoassay for detection of chloramphenicol residue

被引：27

作者：

Xia, Xiaohu ^{[1
]}

Xu, Ye ^{[1
]}

Ke, Rongqin ^{[1
]}

Zhang, Heng ^{[2
]}

Zou, Mingqiang ^{[3
]}

Yang, Wei ^{[1
]}

Li, Qingge ^{[1
]}

机构：

[1] Xiamen Univ, Sch Life Sci, Dept Biomed Sci, Engn Res Ctr Mol Diagnost,Minist Educ, Xiamen 361005, Fujian, Peoples R China

[2] Shenzhen Entry Exit Inspect & Quarantine Bur, Shenzhen 518067, Peoples R China

[3] Chinese Acad Inspect & Quarantine, Beijing 100123, Peoples R China

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2013年 / 405卷 / 23期

基金：

中国国家自然科学基金;

关键词：

Detection; Chloramphenicol; Lateral flow immunoassay; Europium chelate; Nanoparticle; LINKED-IMMUNOSORBENT-ASSAY; SILICA NANOPARTICLES; CHICKEN MUSCLE; MILK; 2002/657/EC; VALIDATION; TISSUES; SHRIMP; HONEY; ELISA;

D O I：

10.1007/s00216-013-7210-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A europium nanoparticle-based lateral flow immunoassay for highly sensitive detection of chloramphenicol residue was developed. The detection result could be either qualitatively resolved with naked eye or quantitatively analyzed with the assistance of a digital camera. In the qualitative mode, the limit of detection (LOD) was found to be 0.25 ng/mL. In the quantitative mode, the half-maximal inhibition concentration (IC50) was determined to be 0.45 ng/mL and the LOD can reach an ultralow level of 0.03 ng/mL, which is similar to 100 times lower than that of the conventional colloidal gold-based lateral flow immunoassay. Potential application of the established method was demonstrated by analyzing representative cow milk samples.

引用

页码：7541 / 7544

页数：4

共 50 条

[31] Rapid detection of fish major allergen parvalbumin using superparamagnetic nanoparticle-based lateral flow immunoassay
Zheng, Cheng
Wang, Xichang
Lu, Ying
Liu, Yuan
FOOD CONTROL, 2012, 26 (02) : 446 - 452
[32] Dual sensitivity enhancement in gold nanoparticle-based lateral flow immunoassay for visual detection of carcinoembryonic antigen
Mahmoudi, Tohid
Shirdel, Behnaz
Mansoori, Behzad
Baradaran, Behzad
ANALYTICAL SCIENCE ADVANCES, 2020, 1 (03): : 161 - 172
[33] Advances in nanoparticle-based lateral flow immunoassay for point-of-care testing
Lou, Doudou
Fan, Lin
Jiang, Tao
Zhang, Yu
VIEW, 2022, 3 (01)
[34] Double Competitive Immunodetection of Small Analyte: Realization for Highly Sensitive Lateral Flow Immunoassay of Chloramphenicol
Sotnikov, Dmitriy, V
Barshevskaya, Lyubov, V
Bartosh, Anastasia, V
Zherdev, Anatoly, V
Dzantiev, Boris B.
BIOSENSORS-BASEL, 2022, 12 (05): : 343
[35] Nanoparticle-based lateral flow biosensors
Quesada-Gonzalez, Daniel
Merkoci, Arben
BIOSENSORS & BIOELECTRONICS, 2015, 73 : 47 - 63
[36] Lateral flow immunoassay based on aggregation induced emission nanobeads for the sensitive and accurate detection of chloramphenicol in pig hair
Lai, Keyang
Xu, Jun
Luo, Kai
Xie, Min
Chen, Yuan
Li, Fan
Zhou, Yaomin
Gong, Lihui
Xiong, Yonghua
Lai, Weihua
ANALYTICAL BIOCHEMISTRY, 2025, 698
[37] Europium Nanoparticle-Based High Performing Immunoassay for the Screening of Treponemal Antibodies
Talha, Sheikh M.
Hytonen, Jukka
Westhorpe, Adam
Kumar, Sushil
Khanna, Navin
Pettersson, Kim
PLOS ONE, 2013, 8 (12):
[38] Gold nanoparticle-based lateral flow strips for rapid and sensitive detection of Virginiamycin M1
Xu, Xiaoxin
Wu, Xiaoling
Kuang, Hua
Song, Shanshan
FOOD AND AGRICULTURAL IMMUNOLOGY, 2020, 31 (01) : 764 - 777
[39] A highly sensitive gold nanoparticle-based electrochemical aptasensor for theophylline detection
Chen, Xifeng
Guo, Zhenzhen
Tang, Yuguo
Shen, Ying
Miao, Peng
ANALYTICA CHIMICA ACTA, 2018, 999 : 54 - 59
[40] Quantum dot-based lateral flow immunoassay for detection of chloramphenicol in milk
Berlina, Anna N.
Taranova, Nadezhda A.
Zherdev, Anatoly V.
Vengerov, Yuri Y.
Dzantiev, Boris B.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2013, 405 (14) : 4997 - 5000

← 1 2 3 4 5 →