Prevalence of Bacillus cereus group in rice and distribution of enterotoxin genes

被引:0
作者
Jang, JH
Lee, NA
Woo, GJ
Park, JH [1 ]
机构
[1] Kyungwon Univ, Dept Food & Bioengn, Songnam 461701, Kyonggi, South Korea
[2] Korea Food & Drug Adm, Seoul 122704, South Korea
关键词
Bacillus cereus group; Bacillus cereus; Bacillus thuringiensis; prevalence in rice; enterotoxin distribution; PCR;
D O I
暂无
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Bacillus cereus group comprising B. cereus, B. thuringiensis, and B. mycoides was differentiated by polymerase chain reaction (PCR) and colony morphology. Prevalence of B. cereus group in rice and distribution of enterotoxin genes were determined as possible food poisoning agents. PCR using primers targeted for gyrB and cry genes could distinguish B. thuringiensis from B. cereus, and B. mycoides was differentiated by rhizoid morphological characteristics on nutrient agar. Among 136 rice and their processed products, prevalence of B. cereus group was 40%. B. cereus group consisted of 54 B. cereus, 11 B. thuringiensis, and 1 B. mycoides. Major isolates were B. cereus, with B. thuringiensis detected up to 10% among edible rice tested. Five enterotoxin genes, hbl, nhe, bceT, entFM, and cytK, were broadly distributed among B. cereus group, especially in B. cereus and B, thuringiensis. Prevalence of B. cereus group in rice and enterotoxin distribution suggest B. thuringiensis and B. cereus are toxigenic strain that should be controlled in rice and its products.
引用
收藏
页码:232 / 237
页数:6
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