A molecular cytogenetic study of chromosome 3 rearrangements in small cell lung cancer: Consistent involvement of chromosome band 3q13.2

被引:20
作者
Dennis, TR [1 ]
Stock, AD [1 ]
机构
[1] Univ Nevada, Sch Med, Dept Pathol, Mol Cytogenet Lab, Reno, NV 89502 USA
关键词
D O I
10.1016/S0165-4608(99)00023-0
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
To more precisely determine the nature of chromosome 3 rearrangements in small cell lung carcinomas (SCLCs), we have applied molecular cytogenetic technologies to a newly characterized SCLC tumor and five SCLC cell lines. Fluorescent in situ hybridization, chromosome microdissection, and, on the previously uncharacterized tumor, spectral karyotyping was utilized to determine chromosome 3 rearrangements. In all cases, our studies were performed on previously G-banded chromosomes in a sequential manner to facilitate a direct comparison. A consistent breakpoint on the long arm of chromosome 3 at band 3q13.2 was identified in all six tumors. This breakpoint was commonly the result of complex chromosomal rearrangements. Loss of the entire short arm of a chromosome 3 was noted in all six tumor cultures. Two of these cell lines had two sublines, one of which contained a 3q13.2 rearrangement and the other of which contained a chromosome rearrangement that resulted in loss of a chromosome 3 short arm. This consistent rearrangement at chromosome band 3q13.2, as demonstrated by molecular cytogenetic methods, may indicate the location of a gene important in the tumorigenesis of SCLC. (C) Elsevier Science Inc., 1999. All rights reserved.
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页码:134 / 140
页数:7
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