Losartan Affects Glomerular AKT and mTOR Phosphorylation in an Experimental Model of Type 1 Diabetic Nephropathy

被引：25

作者：

Mavroeidi, Vasiliki ^{[1
]}

Petrakis, Ioannis ^{[1
]}

Stylianou, Kostas ^{[1
]}

Katsarou, Theodora ^{[1
]}

Giannakakis, Konstantinos ^{[4
]}

Perakis, Kostas ^{[1
]}

Vardaki, Eleftheria ^{[1
]}

Stratigis, Spyridon ^{[1
]}

Ganotakis, Emmanuel ^{[2
]}

Papavasiliou, Stathis ^{[3
]}

Daphnis, Eugenios ^{[1
]}

机构：

[1] Univ Crete, Dept Nephrol, Khania, Greece

[2] Univ Crete, Dept Internal Med, Khania, Greece

[3] Univ Crete, Dept Endocrinol, Khania, Greece

[4] Univ Roma La Sapienza, Dept Radiol Oncol & Pathol, Rome, Italy

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2013年 / 61卷 / 06期

关键词：

losartan; mTOR; AKT; diabetic nephropathy; podocytes; type; 1; diabetes; RENIN-ANGIOTENSIN SYSTEM; MAMMALIAN TARGET; RAPAMYCIN; PATHWAY; DISEASE; CELLS; RATS; PODOCYTES; BLOCKADE; NEPHRIN;

D O I：

10.1369/0022155413482925

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The AKT-mTOR pathway is activated in diabetic nephropathy. Renin-angiotensin system modulators exert beneficial effects on the diabetic kidney. We explored the action of losartan on AKT-mTOR phosphorylation in glomeruli and podocytes. Diabetes mellitus was induced to Sprague-Dawley rats by streptozotocin. Five months later, the rats were commenced on losartan and euthanized 2 months later. Kidneys were processed for immunofluorescence studies. Glomeruli were isolated for Western blot analysis. Diabetes increased activated forms of AKT and mTOR both in glomeruli and podocytes. In diabetic rats, losartan decreased phosphorylated/activated forms of AKT (Thr308) and mTOR (Ser2448) in glomeruli but decreased only activated mTOR in podocytes. However, in both glomeruli and podocytes of healthy animals, an inverse pattern was evident. In conclusion, a new body of evidence indicates the differential activation of AKT-mTOR in glomeruli and podocytes of healthy and diabetic animals in response to losartan.

引用

页码：433 / 443

页数：11

共 27 条

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