Simultaneous Determination of Isollavones, Saponins and Flavones in Flos Puerariae by Ultra Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry

被引:0
作者
Lu, Jing [1 ]
Xie, Yuanyuan [1 ]
Tan, Yao [1 ]
Qu, Jialin [1 ]
Matsuda, Hisashi [2 ]
Yoshikawa, Masayuki [2 ]
Yuan, Dan [1 ]
机构
[1] Shenyang Pharmaceut Univ, Sch Tradit Chinese Med, Shenyang 110016, Peoples R China
[2] Kyoto Pharmaceut Univ, Dept Pharmacognosy, Yamashina Ku, Kyoto 6078412, Japan
关键词
Pueraria flower; isoflavone; saponin; flavone; ultra performance liquid chromatography; quadrupole time-of-flight mass spectrometry; INDUCED DIABETIC-RAT; LOBATA FLOWER; KAIKASAPONIN-III; HPLC; ISOFLAVONES; IDENTIFICATION; GLYCOSIDES; MICE;
D O I
暂无
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
An ultra performance liquid chromatography (UPLC) coupled with quadrupole time-of-flight mass spectrometry (QTOF/MS) method is established for the rapid analysis of isoflavones, saponins and flavones in 16 samples originated from the flowers of Pueraria lobata and P. thomsonii. A total of 25 isoflavones, 13 saponins and 3 flavones were identified by co-chromatography of sample extract with authentic standards and comparing the retention time, UV spectra, characteristic molecular ions and fragment ions with those of authentic standards, or tentatively identified by MS/MS determination along with Mass Fragment software. Moreover, the method was validated for the simultaneous quantification of 29 components. The samples from two Pueraria flowers significantly differed in the quality and quantity of isoflavones, saponins and flavones, which allows the possibility of showing their chemical distinctness, and may be useful in their standardization and quality control. Dataset obtained from UPLC-MS was processed with principal component analysis (PCA) and orthogonal partial least squared discriminant analysis (OPLS-DA) to holistically compare the difference between both Pueraria flowers.
引用
收藏
页码:941 / 951
页数:11
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