Mepanipyrim haptens and antibodies with nanomolar affinity

被引：17

作者：

Esteve-Turrillas, Francesc A. ^{[1
]}

Mercader, Josep V. ^{[1
]}

Agullo, Consuelo ^{[2
]}

Abad-Somovilla, Antonio ^{[2
]}

Abad-Fuentes, Antonio ^{[1
]}

机构：

[1] Consejo Super Invest Cient IATA CSIC, Inst Agrochem & Food Technol, Valencia 46980, Spain

[2] Univ Valencia, Dept Organ Chem, E-46100 Valencia, Spain

来源：

ANALYST | 2013年 / 138卷 / 12期

关键词：

SOLID-PHASE EXTRACTION; GENERATION; CHROMATOGRAPHY; PYRIMETHANIL; WINE;

D O I：

10.1039/c3an00228d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Mepanipyrim is an anilinopyrimidine fungicide used worldwide for crop protection. With the aim of developing useful immunoreagents for mepanipyrim immunoanalysis, two new functionalized derivatives were prepared and antibodies were generated. Affinity and specificity were assessed by direct and indirect competitive ELISA using homologous and heterologous conjugates. Although all antibodies were selective for the target analyte, the immunizing hapten structure was revealed as a determinant for high-affinity antibody production (IC50 = 3 nM).

引用

页码：3360 / 3364

页数：5

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