Comammox Functionality Identified in Diverse Engineered Biological Wastewater Treatment Systems

被引：122

作者：

Annavajhala, Medini K. ^{[1
,3
]}

Kapoor, Vikram ^{[2
,4
]}

Santo-Domingo, Jorge ^{[2
]}

Chandran, Kartik ^{[1
]}

机构：

[1] Columbia Univ, Dept Earth & Environm Engn, 500 W 120th St, New York, NY 10027 USA

[2] US EPA, Off Res & Dev, Cincinnati, OH 45268 USA

[3] Columbia Univ, Dept Med, Div Infect Dis, New York, NY 10032 USA

[4] Univ Texas San Antonio, Dept Civil & Environm Engn, San Antonio, TX 78249 USA

来源：

ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS | 2018年 / 5卷 / 02期

关键词：

AEROBIC GRANULAR SLUDGE; COMPLETE NITRIFICATION; METAGENOMIC ANALYSIS; PARTIAL NITRITATION; OXIDIZING BACTERIA; MICROBIAL ECOLOGY; LOW-TEMPERATURES; NITROUS-OXIDE; ANAMMOX; NITROSPIRA;

D O I：

10.1021/acs.estlett.7b00577

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Complete ammonia oxidation (comammox) to nitrate by certain Nitrospira-lineage bacteria (CMX) could contribute to overall nitrogen cycling in engineered biological nitrogen removal (BNR) processes in addition to the more well-documented nitrogen transformations by ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB), and anaerobic ammonia-oxidizing (anammox) bacteria (AMX). A metagenomic survey was conducted to quantify the presence and elucidate the potential functionality of CMX in 16 full-scale BNR configurations treating mainstream or sidestream wastewater. CMX proposed to date were combined with previously published AOB, NOB, and AMX genomes to create an expanded database for alignment of metagenomic reads. CMX-assigned metagenomic reads accounted for between 0.28 and 0.64% of total coding DNA sequences in all BNR configurations. Phylogenetic analysis of key nitrification functional genes amoA, encoding the alpha-subunit of ammonia monooxygenase, haoB, encoding the beta-subunit of hydroxylamine oxidoreductase, and nxrB, encoding the beta-subunit of nitrite oxidoreductase, confirmed that each BNR system contained coding regions for production of these enzymes by CMX specifically. Ultimately, the ubiquitous presence of CMX bacteria and metabolic functionality in such diverse system configurations emphasizes the need to translate novel bacterial transformations to engineered biological process interrogation, operation, and design.

引用

页码：110 / 116

页数：7

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