Evaluation of generations 2, 3 and 4 arginine modified PAMAM dendrimers for gene delivery

被引：75

作者：

Nam, Hye Yeong ^{[1
]}

Hahn, Hwa Jeong ^{[1
]}

Nam, Kihoon ^{[1
]}

Choi, Woo-Hyung ^{[2
]}

Jeong, Yunseong ^{[1
]}

Kim, Dong-Eun ^{[2
]}

Park, Jong-Sang ^{[1
]}

机构：

[1] Seoul Natl Univ, Sch Chem & Mol Engn, Seoul 151742, South Korea

[2] Konkuk Univ, Dept Biosci & Biotechnol, Seoul 143701, South Korea

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2008年 / 363卷 / 1-2期

关键词：

Biodegradation; PAMAM; Generation; Oligonucleotide; Transfection; Cytotoxicity;

D O I：

10.1016/j.ijpharm.2008.07.021

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

It is a matter of concern to develop and design synthetic non-viral gene carriers with high transfection efficiency and low cytotoxicity in gene therapy. Recently, various arginine conjugated dendrimers showed better performance in transfection and greater viability than polyethyleneimine (PEI). In this study, we synthesized and investigated e-PAM-R G2, 3 and 4 which are biodegradable polyamidoamine (PAMAM) dendrimers modified with arginine and compared that with PAMAM-R series containing amide bonds for gene carriers. For plasmid DNA delivery, the transfection efficiency of e-PAM-R G4 was higher than G3 and G2 and similar to PAMAM-R G4 with favorable cell viability. Moreover, they indicated significantly higher suppression of TEL/AML1 protein, maybe due to rapid olidonucleotide (ODNs) release through biodegradability of e-PAM-R. These results suggest that biodegradable and non-toxic e-PAM-R may be useful carriers for the gene including plasmid DNA, antisense ODNs and si-RNA. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：199 / 205

页数：7

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