Cyclic strain dominates over microtopography in regulating cytoskeletal and focal adhesion remodeling of human mesenchymal stem cells

被引：13

作者：

Doroudian, Golnar ^{[1
]}

Curtis, Matthew W. ^{[1
,2
]}

Gang, Anjulie ^{[2
]}

Russell, Brenda ^{[2
]}

机构：

[1] Univ Illinois, Dept Bioengn, Chicago, IL 60612 USA

[2] Univ Illinois, Dept Physiol & Biophys, Chicago, IL 60612 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2013年 / 430卷 / 03期

基金：

美国国家卫生研究院;

关键词：

Micromechanics; Microarray; Adhesion; Migration; EXPRESSION; MECHANOTRANSDUCTION; PROLIFERATION; DIFFERENTIATION; RHOA/ROCK; STRETCH;

D O I：

10.1016/j.bbrc.2012.11.120

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human bone marrow-derived mesenchymal stem cell (hMSCs) function depends on chemical factors and also on the physical cues of the microenvironmental niche. Here, this physical microenvironment is recapitulated with controlled modes of mechanical strain applied to substrata containing three-dimensional features in order to analyze the effects on cell morphology, focal adhesion distribution, and gene expression. Ten percentage of strain at 1 Hz is delivered for 48 h to hMSCs cultured on flat surfaces, or on substrata with 15 mu m-high microtopographic posts spaced 75 mu m apart. Adding strain to microtopography produced stable semicircular focal adhesions, and actin spanning from post to post. Strain dominated over microtopography for expression of genes for the cytoskeleton (caldesmon-1 and calponin 3), cell adhesion (integrin-alpha 2, vinculin, and paxillin), and extracellular matrix remodeling (MMP13) (p < 0.05). Overall, attention to external mechanical stimuli is necessary for optimizing the stem cell niche for regenerative medicine. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：1040 / 1046

页数：7

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