Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil

被引：28

作者：

Lemos, Tatiana Didonet ^{[1
]}

Figueiredo Cerqueira, Aloysio de Mello ^{[2
]}

Toma, Helena Keiko ^{[3
]}

da Silva, Adrianna Vieira ^{[4
]}

Bartolomeu Correa, Rafael Gomes ^{[4
]}

Paludo, Giane Regina ^{[5
]}

Massard, Carlos Luiz ^{[6
]}

Pereira Almosny, Nadia Regina ^{[1
]}

机构：

[1] Univ Fed Fluminense UFF, Dept Patol & Clin Vet, Niteroi, RJ, Brazil

[2] Univ Fed Fluminense UFF, Dept Microbiol & Parasitol, Niteroi, RJ, Brazil

[3] Univ Fed Rio de Janeiro UFRJ, Fac Farm, Lab Diagnost Mol & Hematol, Rio De Janeiro, RJ, Brazil

[4] VetLab Anal Clin, Rio De Janeiro, RJ, Brazil

[5] Univ Brasilia UnB, Fac Agron & Med Vet, Brasilia, DF, Brazil

[6] Univ Fed Rural Rio de Janeiro UFRRJ, Dept Parasitol Anim, Rio De Janeiro, RJ, Brazil

来源：

REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA | 2012年 / 21卷 / 02期

关键词：

Babesia vogeli; Rangelia vitalii; piroplasms; dogs; PCR; BABESIA-CANIS-CANIS; VOGELI; THEILERIA; CONRADAE; SUBUNIT; PCR;

D O I：

10.1590/S1984-29612012000200012

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Rangelia vitalii is a protozoon described from dogs in the south and southeast regions of Brazil. Iris phylogenetically related to Babesia spp. that infects dogs, but data on this enigmatic parasite is still limited. The aim of this work was to detect piroplasm species in dogs in the state of Rio de Janeiro, Brazil, by 18S rRNA gene-based PCR assay, restriction fragment length polymorphism (RFLP) and sequence analyses. Of 103 dogs examined, seven (6.8%) were positive for Babesia spp. by PCR. The amplified products were digested by restriction enzymes to differentiate the Babesia species, and one sample was identified as Babesia vogeli. The pattern observed for the other six amplification products did not match with pattern described for large Babesia infecting dogs. Sequencing analysis confirmed these six samples as R. vitalii, with high homologies (99-100%) with a sequence from south Brazil. This study confirms the presence of Babesia vogeli and Rangelia vitalii circulate in domestic dogs in Teresopolis, Rio de Janeiro, Brazil.

引用

页码：137 / 142

页数：6

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